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Recombinant Saccharomyces cerevisiae Transcription factor IIIB 70 kDa subunit (BRF1), partial

  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Transcription factor IIIB 70 kDa subunit(BRF1) ,partial
  • 貨號:
    CSB-YP326852SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Transcription factor IIIB 70 kDa subunit(BRF1) ,partial
  • 貨號:
    CSB-EP326852SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Transcription factor IIIB 70 kDa subunit(BRF1) ,partial
  • 貨號:
    CSB-EP326852SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Transcription factor IIIB 70 kDa subunit(BRF1) ,partial
  • 貨號:
    CSB-BP326852SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Transcription factor IIIB 70 kDa subunit(BRF1) ,partial
  • 貨號:
    CSB-MP326852SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    BRF1
  • Uniprot No.:
  • 別名:
    BRF1; PCF4; TDS4; YGR246C; Transcription factor IIIB 70 kDa subunit; TFIIIB; B-related factor 1; BRF-1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.
  • 基因功能參考文獻:
    1. Data indicate the binding between Brf1 cyclin repeats and the highly conserved region connecting C34 winged-helix domains 2 and 3. PMID: 24277937
    2. analysis of the interaction site of the Brf1 and Bdp1 subunits of Saccharomyces cerevisiae TFIIIB PMID: 16551611
    3. Entry of Brf1 and Bdp1 into the transcription complex imposes a strict sequence preference for the downstream half of the TATA box that matches the TATA box of the pol III-transcribed U6 small nuclear RNA gene. PMID: 17028095
    4. A structural model of the interaction interface between transcription factor TFIIIB essential subunit (Bdp1) and Brf1 shows the main Bdp1 anchorage site provided by a small set of residues belonging to a Brf1 segment of residues 470-495. PMID: 19086269
  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    TFIIB family
  • 數據庫鏈接:

    KEGG: sce:YGR246C

    STRING: 4932.YGR246C



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