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  4. Recombinant Saccharomyces cerevisiae Transcription elongation factor SPT4 (SPT4)

Recombinant Saccharomyces cerevisiae Transcription elongation factor SPT4 (SPT4)

  • 中文名稱：
    釀酒酵母SPT4重組蛋白
  • 貨號(hào)：
    CSB-YP022957SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SPT4重組蛋白
  • 貨號(hào)：
    CSB-EP022957SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SPT4重組蛋白
  • 貨號(hào)：
    CSB-EP022957SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SPT4重組蛋白
  • 貨號(hào)：
    CSB-BP022957SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SPT4重組蛋白
  • 貨號(hào)：
    CSB-MP022957SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SPT4
  • Uniprot No.：
  • 別名：
    SPT4; YGR063C; Transcription elongation factor SPT4; Chromatin elongation factor SPT4
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-102
  • 氨基酸序列
    MSSERACMLC GIVQTTNEFN RDGCPNCQGI FEEAGVSTME CTSPSFEGLV GMCKPTKSWV AKWLSVDHSI AGMYAIKVDG RLPAEVVELL PHYKPRDGSQ VE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. The complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene. Structural and functional component of the centromeric and heterochromatic loci linking chromatin structure with kinetochore function and gene silencing.
  • 基因功能參考文獻(xiàn)：
    1. Interactions between SPT4 and RNA Polymerase II PMID: 27261007
    2. Spt4/5 may be important to coordinate the mechanical movement of RNAPII through the nucleosome with co-transcriptional chromatin modifications during transcription. PMID: 28379497
    3. These findings attribute a new function to a domain of Spt4/5 that associates directly with RNAPII, making significant steps towards elucidating the mechanism behind transcriptional control by Spt4/5. PMID: 27376968
    4. demonstrate that physical and genetic interactions exist between Hot1 and several proteins involved in transcriptional and posttranscriptional processes: for example, transcription co-activator Sub1 and elongation complex Spt4/5 PMID: 27480106
    5. Study reports that transcription of genes containing long repeats of CAG or other trinucleotides located in either protein-coding or transcribed noncoding regions of templates is selectively facilitated by the actions of Spt4 PMID: 22341442
    6. Data provide evidence implicating the yeast DSIF complex (Spt4/5) and RNA polymerase II phosphorylation by Kin28 and Ctk1 in the recruitment of Rpd3S to active genes. PMID: 21060864
    7. She2p interacts in vivo with the elongating forms of RNA polymerase II (pol II) via the transcription elongation factor Spt4-Spt5 PMID: 20713510
    8. one function of Spt4-Spt5 is to help RNA polymerase II overcome the repressive effects of these histone modifications and chromatin regulators on transcription PMID: 19948887
    9. Absence of functional Spt4p leads to altered chromatin structure at the centromeric DNA and mislocalization of the mammalian CENP-A homolog Cse4p to noncentromeric loci PMID: 15057281
    10. Spt4-Spt5 complex may help coordinate splicing with transcription under conditions that present kinetic challenges to spliceosome assembly or function PMID: 16172632
    11. data suggest that Spt4p, Spt5p, and, potentially, other regulators of Pol I transcription elongation play important roles in coupling rRNA transcription to its processing and ribosome assembly PMID: 16908835
    12. Structural and evolutionary perspective of Spt4-Spt5 complex and suggests that it is an ancient, core component of the transcription elongation machinery. PMID: 19000817

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome, centromere. Note=Centromere and heterochromatin.
  • 蛋白家族：
    SPT4 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YGR063C

    STRING: 4932.YGR063C



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