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Recombinant Saccharomyces cerevisiae Sterol 24-C-methyltransferase (ERG6)

  • 中文名稱:
    釀酒酵母ERG6重組蛋白
  • 貨號:
    CSB-YP326516SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母ERG6重組蛋白
  • 貨號:
    CSB-EP326516SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母ERG6重組蛋白
  • 貨號:
    CSB-EP326516SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母ERG6重組蛋白
  • 貨號:
    CSB-BP326516SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母ERG6重組蛋白
  • 貨號:
    CSB-MP326516SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    ERG6
  • Uniprot No.:
  • 別名:
    ERG6; ISE1; LIS1; SED6; YML008C; YM9571.10CSterol 24-C-methyltransferase; EC 2.1.1.41; Delta(24)-sterol C-methyltransferase
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Full Length of Mature Protein
  • 表達區域:
    2-383
  • 氨基酸序列
    SETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW DGRTDKDAEE RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF AASIARHEHY LAYKAGIQRG DLVLDVGCGV GGPAREIARF TGCNVIGLNN NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK MDFEENTFDK VYAIEATCHA PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK IAYEIELGDG IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA GGKSKLFTPM MLFVARKPEN AETPSQTSQE ATQ
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.
  • 基因功能參考文獻:
    1. Lis1 has two opposing modes of regulating dynein, being capable of inducing both low and high affinity for the microtubule; study shows that these opposing modes depend on the stoichiometry of Lis1 binding to dynein and that this stoichiometry is regulated by the nucleotide state of dynein's AAA3 domain. PMID: 28886386
    2. Data show that the erg6Delta mutant strain is more sensitive to hyperosmotic shocks than is WT. PMID: 21081111
    3. Erg28p may not only anchor the C-4 demethylation enzyme complex to the ER but also acts as a protein bridge to the Erg6p enzyme required for the next ergosterol biosynthetic step. PMID: 15522820
    4. photolabeling & mutagenesis were performed on 24-C-sterol methyltransferase to elucidate location & role of active site residues involved in AdoMet binding & catalysis; results indicate that one or both of Cys128 & Pro133 are covalently bound to AdoMet PMID: 18563465
    5. Overexpression of ERG6 and ERG2 led to an increased ergosterol level. PMID: 19835168

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  • 蛋白家族:
    Class I-like SAM-binding methyltransferase superfamily, Erg6/SMT family
  • 數據庫鏈接:

    KEGG: sce:YML008C

    STRING: 4932.YML008C



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