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  4. Recombinant Saccharomyces cerevisiae Spindle assembly checkpoint component MAD1 (MAD1), partial

Recombinant Saccharomyces cerevisiae Spindle assembly checkpoint component MAD1 (MAD1), partial

  • 中文名稱(chēng)：
    釀酒酵母MAD1重組蛋白
  • 貨號(hào)：
    CSB-YP334793SVG
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母MAD1重組蛋白
  • 貨號(hào)：
    CSB-EP334793SVG
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母MAD1重組蛋白
  • 貨號(hào)：
    CSB-EP334793SVG-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母MAD1重組蛋白
  • 貨號(hào)：
    CSB-BP334793SVG
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母MAD1重組蛋白
  • 貨號(hào)：
    CSB-MP334793SVG
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MAD1
  • Uniprot No.：
  • 別名：
    MAD1; YGL086WSpindle assembly checkpoint component MAD1; Mitotic arrest deficient protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Central component of the spindle assembly checkpoint. Thought to recruit MAD2 to unattached kinetochores. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism.
  • 基因功能參考文獻(xiàn)：
    1. Mad1 recruitment to the kinetochore and it's role in the spindle assembly checkpoint PMID: 27280788
    2. Mad1 is recruited by detached kinetochores competing with alternate other sites in nucleus: Mad1 pool is small enough to be fully depleted by one pair of detached kinetochores. Lateral attachment licenses Mad1 removal from kinetochores after kinetic delay PMID: 26023090
    3. Mad1p senses unattached kinetochores and, by altering nuclear pore complex transport activity, regulates the nuclear environment of the spindle. PMID: 23177738
    4. cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension. PMID: 21593209
    5. Saccharomyces cerevisiae Mad1p C-terminal domain mediates spindle checkpoint function, chromosome transmission fidelity and CEN association PMID: 15802513
    6. Only the C terminus is required for spindle assembly checkpoint (SAC) function and the nuclear pore complex, through Nup53p, may act to regulate the duration of the SAC response. PMID: 16000377

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    MAD1 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YGL086W

    STRING: 4932.YGL086W



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