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  4. Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase KIN28 (KIN28)

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase KIN28 (KIN28)

  • 中文名稱：
    釀酒酵母KIN28重組蛋白
  • 貨號：
    CSB-YP361758SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母KIN28重組蛋白
  • 貨號：
    CSB-EP361758SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母KIN28重組蛋白
  • 貨號：
    CSB-EP361758SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母KIN28重組蛋白
  • 貨號：
    CSB-BP361758SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母KIN28重組蛋白
  • 貨號：
    CSB-MP361758SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    KIN28
  • Uniprot No.：
  • 別名：
    KIN28; YDL108W; D2330Serine/threonine-protein kinase KIN28; EC 2.7.11.23
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-306
  • 氨基酸序列
    MKVNMEYTKE KKVGEGTYAV VYLGCQHSTG RKIAIKEIKT SEFKDGLDMS AIREVKYLQE MQHPNVIELI DIFMAYDNLN LVLEFLPTDL EVVIKDKSIL FTPADIKAWM LMTLRGVYHC HRNFILHRDL KPNNLLFSPD GQIKVADFGL ARAIPAPHEI LTSNVVTRWY RAPELLFGAK HYTSAIDIWS VGVIFAELML RIPYLPGQND VDQMEVTFRA LGTPTDRDWP EVSSFMTYNK LQIYPPPSRD ELRKRFIAAS EYALDFMCGM LTMNPQKRWT AVQCLESDYF KELPPPSDPS SIKIRN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalytic component of the TFIIK complex (KIN28-CCL1 dimer) which is the protein kinase component of transcription factor IIH (TFIIH) and phosphorylates the C-terminal domain of RNA polymerase II during transition from transcription to elongation after preinitiation complex (PIC) formation, thereby positively regulating transcription. TFIIH (or factor B) is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has DNA-dependent ATPase activity and is essential for polymerase II transcription in vitro. Essential for cell proliferation.
  • 基因功能參考文獻：
    1. Inactivating Kin28 impacts promoter release to differing degrees and reveals a "checkpoint" during the transition to productive elongation, resulting in widespread mRNA stabilization. PMID: 27477907
    2. Kin28 has a role in the association of Mediator with promoters. PMID: 24704787
    3. These results identify an Spt5 phosphorylated C-terminal repeats-independent pathway of Paf1C recruitment requiring Kin28, Bur1, and the Spt5 phosphorylated C-terminal repeats-interaction domain of Cdc73. PMID: 22796944
    4. These results suggest that the overexpression of Kin28 and Pog1 enhances the protein refolding and degradation pathways in rsp5(A401E) cells. PMID: 17986087
    5. Results implicate the TFIIH kinase in placing bivalent Ser5 and Ser7 marks early in gene transcription. PMID: 19450536
    6. Study points strongly toward a minor role of TFIIH kinase in Pol II transcription, and a more significant role in mRNA capping in budding yeast. PMID: 19666497

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 數據庫鏈接：

    KEGG: sce:YDL108W

    STRING: 4932.YDL108W



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