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  4. Recombinant Saccharomyces cerevisiae SAGA-associated factor 73 (SGF73), partial

Recombinant Saccharomyces cerevisiae SAGA-associated factor 73 (SGF73), partial

  • 中文名稱：
    釀酒酵母SGF73重組蛋白
  • 貨號：
    CSB-YP347386SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SGF73重組蛋白
  • 貨號：
    CSB-EP347386SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SGF73重組蛋白
  • 貨號：
    CSB-EP347386SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SGF73重組蛋白
  • 貨號：
    CSB-BP347386SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SGF73重組蛋白
  • 貨號：
    CSB-MP347386SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SGF73
  • Uniprot No.：
  • 別名：
    SGF73; YGL066W; SAGA-associated factor 73; 73 kDa SAGA-associated factor; SAGA histone acetyltransferase complex 73 kDa subunit
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs).
  • 基因功能參考文獻：
    1. The replicative lifespan-extending deletion of SGF73 results in altered ribosomal gene expression. PMID: 28568901
    2. The 3D structure of SAGA complexes in which the subunit Sgf73 is partially or fully deleted to remove the deubiquitination module revealed the module localized in the vicinity of Gcn5 and Spt7. PMID: 25441028
    3. The Sgf73 subunit of the SAGA deubiquitinating module (DUBm) activates the catalytic Ubp8 subunit. PMID: 25526805
    4. Sgf73 region (373-402 aa) is essential for heterochromatin boundary function. PMID: 23819448
    5. Solution NMR and circular dichroism analysis of Sgf73(1-104) after zinc ion removal using chelation reagent EDTA demonstrated that zinc ion was required to maintain stable conformation of the zinc finger motif. PMID: 20510875
    6. study reports the 1.90 angstrom resolution crystal structure of the deubiquitinating module (DUBm)- which contains Ubp8 bound to Sgf11, Sus1 and Sgf73- bound to ubiquitin aldehyde, as well as the 2.45 angstrom resolution structure of the uncomplexed DUBm PMID: 20395473

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Ataxin-7 family
  • 數據庫鏈接：

    KEGG: sce:YGL066W

    STRING: 4932.YGL066W



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