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  4. Recombinant Saccharomyces cerevisiae S-phase entry cyclin-5 (CLB5)

Recombinant Saccharomyces cerevisiae S-phase entry cyclin-5 (CLB5)

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae S-phase entry cyclin-5(CLB5)
  • 貨號：
    CSB-YP341136SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae S-phase entry cyclin-5(CLB5)
  • 貨號：
    CSB-EP341136SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae S-phase entry cyclin-5(CLB5)
  • 貨號：
    CSB-EP341136SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae S-phase entry cyclin-5(CLB5)
  • 貨號：
    CSB-BP341136SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae S-phase entry cyclin-5(CLB5)
  • 貨號：
    CSB-MP341136SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CLB5
  • Uniprot No.：
  • 別名：
    CLB5; YPR120C; P9642.8S-phase entry cyclin-5
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-435
  • 氨基酸序列
    MGENHDHEQS IKRNSMIYNE NERQLCNSNL KILQNKRALS KNDSSSKQQV QDSKPRRALT DVPVNNNPLS QNKRIVAGSK AAKVRREENI RPIVSAVQKR QIYNDRTAAE QEEEEEEEGE DDDAASIVNK KRRIDAEGVS EIVGWQDLDY VEKDDTAMVA EYSAEIFAFL YRRELETLPS HNYLLDKTSK YYLRPSMRTI LVDWLVEVHE KFQCYPETLF LSINLMDRFL AKNKVTMNKL QLLAVTSLFI AAKFEEVNLP KLAEYAYITD GAASKNDIKN AEMFMLTSLE FNIGWPNPLN FLRRISKADD YDPVNRNIGK FILEYAYCCH QFIHLPPSTV SAMAMYIARR MTNRNKNELW NGTLQHYSGG IDPIHDEAFQ SLCIDLVKDI ASSKTHLDSL ILKYKKPRYG SVYFQTFKWC TSEMHSNFQN LFNLK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for efficient progression through S phase and possibly for the normal progression through meiosis. Interacts with CDC28.
  • 基因功能參考文獻：
    1. Results suggest that Clb5 has a unique ability to trigger premeiotic S phase and that the amino-terminal region of Clb5 contributes to its specificity and regulates the functions performed by the cyclin-Cdk complex. PMID: 22209902
    2. The lack of Snf1 alpha-catalytic subunit down-regulates CLB5 expression. PMID: 20505334
    3. In the presence of replication stress, the unstable Clb5 pool is stabilized, and such stabilization requires neither MBF transcriptional activity nor de novo protein synthesis. PMID: 20538605
    4. The 'hydrophobic patch' (HP) of the Saccharomyces cerevisiae S-phase cyclin Clb5 facilitates its interaction with Orc6 (via its Cy or RXL motif), providing a mechanism that helps prevent rereplication from individual origins. PMID: 15611618
    5. Activation of CLB5 during meiosis requires Clb5 activity, suggesting that CLB5 may be regulated by a positive feedback mechanism. PMID: 15654101
    6. Absence of the B-type cyclin Clb5 leads to a delay of premeiotic S phase by separate mechanisms. PMID: 16254243
    7. Clb5 is stable until mitosis PMID: 16508019
    8. Phosphorylation of Fin1 from S phase to metaphase, by the cyclin-dependent kinase Clb5-Cdk1, inhibits Fin1 association with the spindle. In anaphase, when Clb5-Cdk1 is inactivated, Fin1 is dephosphorylated by the phosphatase Cdc14. PMID: 17173039
    9. Clb5 proteolysis involves two different modes for targeting of Clb5 to the proteasome, an APC/C-dependent and an APC/C-independent mechanism. PMID: 17620341
    10. The CLB5 promoter and have identified two middle sporulation elements (MSEs) that act as binding sites for Ndt80 and both MSE display differential effectiveness in their ability to function as cis-acting regulatory sequences in vivo. PMID: 18327887
    11. The coincident destruction of securin, Clb5, and Dbf4 coordinates bulk chromosome segregation with segregation of ribosomal DNA. PMID: 18591250
    12. The temporal program of chromosome replication: genomewide replication in clb5{Delta} Saccharomyces cerevisiae PMID: 18832352

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  • 蛋白家族：
    Cyclin family, Cyclin AB subfamily
  • 數據庫鏈接：

    KEGG: sce:YPR120C

    STRING: 4932.YPR120C



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