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Recombinant Saccharomyces cerevisiae Ribonuclease H2 subunit A (RNH201)

  • 中文名稱:
    釀酒酵母RNH201重組蛋白
  • 貨號:
    CSB-YP019805SVG
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母RNH201重組蛋白
  • 貨號:
    CSB-EP019805SVG
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母RNH201重組蛋白
  • 貨號:
    CSB-EP019805SVG-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母RNH201重組蛋白
  • 貨號:
    CSB-BP019805SVG
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母RNH201重組蛋白
  • 貨號:
    CSB-MP019805SVG
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    RNH201
  • Uniprot No.:
  • 別名:
    RNH201; RNH35; YNL072W; N2369; Ribonuclease H2 subunit A; RNase H2 subunit A; EC 3.1.26.4; RNase H(201); RNase H(35); Ribonuclease HI large subunit; RNase HI large subunit; Ribonuclease HI subunit A
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區(qū)域:
    1-307
  • 氨基酸序列
    MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM VYAVAYSTQK YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI GYATTCITPL DISRGMSKFP PTRNYNLNEQ AHDVTMALID GVIKQNVKLS HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK KADSLYCMVS VASVVAKVTR DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP ANMVRFSWQT CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR TLDNWYR
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
  • 基因功能參考文獻:
    1. Both R-loop removal and RER activities of RNase H2 contribute substantially to chromosome stability, and that their relative contributions may be variable across different regions of the genome. In this scenario, a prominent contribution of R-loop removal may be expected at highly transcribed regions, whereas RER may play a greater role at hotspots of ribonucleotide incorporation. PMID: 28268090
    2. An important role for RNase H2 in maintaining genome integrity by removing single ribonucleotides incorporated during DNA replication is shown. PMID: 28325498
    3. Deleting RNH201 elicits a strong cellular stress response in S. cerevisiae. PMID: 23079308
    4. study presents a complete biochemical reconstitution of the ribonucleotide excision repair (RER) pathway; RER is most efficient when the ribonucleotide is incised by RNase H2 PMID: 22864116
  • 亞細胞定位:
    Cytoplasm. Nucleus.
  • 蛋白家族:
    RNase HII family, Eukaryotic subfamily
  • 數(shù)據(jù)庫鏈接:

    KEGG: sce:YNL072W

    STRING: 4932.YNL072W



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