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  4. Recombinant Saccharomyces cerevisiae Repressor of RNA polymerase III transcription MAF1 (MAF1)

Recombinant Saccharomyces cerevisiae Repressor of RNA polymerase III transcription MAF1 (MAF1)

  • 中文名稱：
    釀酒酵母MAF1重組蛋白
  • 貨號：
    CSB-YP340457SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MAF1重組蛋白
  • 貨號：
    CSB-EP340457SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MAF1重組蛋白
  • 貨號：
    CSB-EP340457SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MAF1重組蛋白
  • 貨號：
    CSB-BP340457SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MAF1重組蛋白
  • 貨號：
    CSB-MP340457SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MAF1
  • Uniprot No.：
  • 別名：
    MAF1; YDR005C; YD8119.11CRepressor of RNA polymerase III transcription MAF1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-395
  • 氨基酸序列
    MKFIDELDIE RVNQTLNFET NDCKIVGSCD IFTTKAVASD RKLYKTIDQH LDTILQENEN YNATLQQQLA APETNQSPCS SPFYSNRRDS NSFWEQKRRI SFSEYNSNNN TNNSNGNSSN NNNYSGPNGS SPATFPKSAK LNDQNLKELV SNYDSGSMSS SSLDSSSKND ERIRRRSSSS ISSFKSGKSS NNNYSSGTAT NNVNKRRKSS INERPSNLSL GPFGPINEPS SRKIFAYLIA ILNASYPDHD FSSVEPTDFV KTSLKTFISK FENTLYSLGR QPEEWVWEVI NSHMTLSDCV LFQYSPSNSF LEDEPGYLWN LIGFLYNRKR KRVAYLYLIC SRLNSSTGEV EDALAKKPQG KLIIDDGSNE YEGEYDFTYD ENVIDDKSDQ EESLQ
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Mediator of diverse signals that repress RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.
  • 基因功能參考文獻(xiàn)：
    1. correlation between CCA synthesis and Pol III regulation by Maf1 could be important in coordination of tRNA transcription, processing and regulation of translation PMID: 27575455
    2. Sub1 and Maf1 also exhibit an opposite role on yeast chronological life span PMID: 25531541
    3. PKA modulates Maf1 protein abundance for stress and lifespan but not growth PMID: 25720796
    4. Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway. PMID: 22919049
    5. Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation. PMID: 22810236
    6. Maf1-mediated transcription control and nuclear export by Los1 are two major stages of tRNA biosynthesis that are regulated by environmental conditions in a coordinated manner. PMID: 21940626
    7. an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation. PMID: 21383183
    8. Study reports the crystal structure of Maf1 and cryo-electron microscopic structures of Pol III, an active Pol III-DNA-RNA complex, and a repressive Pol III-Maf1 complex. PMID: 20887893
    9. The authors show that TORC1 interacts with Maf1 in vivo and phosphorylates Maf1 in vitro, and regulates Maf1 nucleoplasm-to-nucleolus translocation. PMID: 19574957
    10. Maf1 does not differentially sequester Brf1 or pol III under repressing conditions. The results suggest that Maf1 functions by a non-stoichiometric mechanism to repress pol III transcription PMID: 15590667
    11. Under repressing conditions, Maf1 is dephosphorylated and accumulates in the nucleus, and Pol III-Maf1 interaction increases. PMID: 16762835
    12. Dephosphorylation and genome-wide association of Maf1 with Pol III-transcribed genes during repression PMID: 16762836
    13. Nuclear accumulation of Maf1 is required for transcriptional repression and is regulated by two nuclear localization sequences in the protein. PMID: 17005718
    14. Transcription regulation by Maf1 is not limited to stress but is important for the switch between fermentation and respiration. PMID: 17785443
    15. phosphorylation of Maf1 inside the nucleus acts both directly by decreasing of Maf1-mediated repression of Pol III and indirectly by stimulation of Msn5 binding and export of nuclear Maf1 to the cytoplasm PMID: 18445601
    16. Maf1 functions in parallel pathways to negatively regulate ribosomal protein mRNA and tRNA synthesis. PMID: 18604275

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    MAF1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YDR005C

    STRING: 4932.YDR005C



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