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  4. Recombinant Saccharomyces cerevisiae Regulatory protein SWI6 (SWI6), partial

Recombinant Saccharomyces cerevisiae Regulatory protein SWI6 (SWI6), partial

  • 中文名稱：
    釀酒酵母SWI6重組蛋白
  • 貨號：
    CSB-YP362844SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SWI6重組蛋白
  • 貨號：
    CSB-EP362844SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SWI6重組蛋白
  • 貨號：
    CSB-EP362844SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SWI6重組蛋白
  • 貨號：
    CSB-BP362844SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SWI6重組蛋白
  • 貨號：
    CSB-MP362844SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SWI6
  • Uniprot No.：
  • 別名：
    SWI6; YLR182W; L9470.8; Regulatory protein SWI6; Cell-cycle box factor subunit SWI6; MBF subunit P90; Trans-acting activator of HO endonuclease gene
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Part of a complex involved in cell-cycle-dependent transcription. SWI4 and SWI6 are required for formation of the cell-cycle box factor-DNA complex. The repeated element in the upstream region of HO (5'-CACGAAAA-3') is called the cell cycle box (CCB).
  • 基因功能參考文獻：
    1. Swi4/Swi6 (SBF) factor binding at HO follows a temporal cascade, with SBF bound at upstream regulatory sequence (URS2-L) serving to relay a signal from URS1 to the SBF sites in URS2-R that ultimately activate gene expression. PMID: 25512608
    2. study identifies a region of the carboxy terminus of Swi6 that is required for Nrm1 and Whi5 binding to their target transcription factors and show that mutation of this region overrides the repression of MBF- and SBF-regulated genes by Nrm1 and Whi5. PMID: 23382076
    3. We found that the transcriptional cofactor Swi6p and activator Swi4p, as well as the G(2)/M-specific cyclin Clb2p, are required in a microtubule-destabilizing environment PMID: 22037179
    4. oxidative stress signaling for cell cycle regulation occurs through oxidation of the G(1)/S-specific transcription factor Swi6p and consequently leads to suppression of the expression of G(1) cyclins and a delay in cells entering the cell cycle. PMID: 21147769
    5. Data show that in Swi6 the structural and physical features of the nucleic acid binding interface have been completely eradicated. PMID: 20635421
    6. Data show that Mpk1 negatively regulates Swi6 by phosphorylation on Ser238, which inhibits nuclear entry. PMID: 20219973
    7. binding of the Swi4/Swi6 (SBF) transcription factors to an evolutionarily conserved site within the recombination enhancer PMID: 16809780
    8. Swi6p is involved in oxidant sensing leading to cell division delay. PMID: 18205808
    9. propose a model in which phosphorylated Mpk1 or Mlp1 forms a dimeric complex with Swi4 that is competent to associate with the FKS2 promoter PMID: 18268013
    10. Identified the cell cycle-regulated SBF protein complex(Swi4/Swi6) as required for H3K79 dimethylation. PMID: 19682934

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  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 數據庫鏈接：

    KEGG: sce:YLR182W

    STRING: 4932.YLR182W



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