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  4. Recombinant Saccharomyces cerevisiae Ras-related protein SEC4 (SEC4)

Recombinant Saccharomyces cerevisiae Ras-related protein SEC4 (SEC4)

  • 中文名稱：
    釀酒酵母SEC4重組蛋白
  • 貨號：
    CSB-YP362164SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SEC4重組蛋白
  • 貨號：
    CSB-EP362164SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SEC4重組蛋白
  • 貨號：
    CSB-EP362164SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SEC4重組蛋白
  • 貨號：
    CSB-BP362164SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SEC4重組蛋白
  • 貨號：
    CSB-MP362164SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SEC4
  • Uniprot No.：
  • 別名：
    SEC4; SRO6; YFL005WRas-related protein SEC4; Suppressor of RHO3 protein 6
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-215
  • 氨基酸序列
    MSGLRTVSAS SGNGKSYDSI MKILLIGDSG VGKSCLLVRF VEDKFNPSFI TTIGIDFKIK TVDINGKKVK LQLWDTAGQE RFRTITTAYY RGAMGIILVY DVTDERTFTN IKQWFKTVNE HANDEAQLLL VGNKSDMETR VVTADQGEAL AKELGIPFIE SSAKNDDNVN EIFFTLAKLI QEKIDSNKLV GVGNGKEGNI SINSGSGNSS KSNCC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in exocytosis. Maybe by regulating the binding and fusion of secretory vesicles with the cell surface. The GTP-bound form of SEC4 may interact with an effector, thereby stimulating its activity and leading to exocytotic fusion. SEC4 may be an upstream activator of the 19.5S SEC8/SEC15 particle. SEC4 probably interacts directly with SEC8; it could serve as the attachment site for the SEC8/SEC15 particle.
  • 基因功能參考文獻：
    1. Data show that mutations in Rab GTPase Sec4p (SEC4) disrupt actin patch assembly and proper endocytic internalization. PMID: 27526190
    2. Sec4p phosphorylation facilitates the coordination of membrane-trafficking events during cytokinesis. PMID: 27621363
    3. Authors describe the identification of a Sec4 binding site on the surface of Sro7 that is contained within a cleft created by the junction of two adjacent beta-propellers that form the core structure of Sro7. PMID: 26202462
    4. Results show that Sec4p serine 29 forms a hydrogen bond with the nucleotide and its can form stable associations with Sec2p allowing to elucidate the structure of the Sec4p-Sec2p complex representing an early stage of the exchange reaction. PMID: 26263895
    5. Intracellular traffic in yeast between the Golgi and the cell surface is mediated by vesicular carriers and highly dependent on both Sro7 and Rab GTPase, Sec4. PMID: 25404740
    6. Data suggest that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation. PMID: 20444978
    7. Sec2 is a highly active exchange factor, in contrast to other proteins previously characterized as Rab exchange factors. PMID: 17134721
    8. The crystal structure of the Sec2p guanine nucleotide exchange factor domain in a complex with the nucleotide-free Sec4p at 2.7 A resolution, is reported. PMID: 17488829

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  • 亞細胞定位：
    Cytoplasmic vesicle, secretory vesicle membrane; Lipid-anchor; Cytoplasmic side. Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm. Note=A small fraction is soluble.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 數據庫鏈接：

    KEGG: sce:YFL005W

    STRING: 4932.YFL005W



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