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  4. Recombinant Saccharomyces cerevisiae Rab guanine nucleotide exchange factor SEC2 (SEC2), partial

Recombinant Saccharomyces cerevisiae Rab guanine nucleotide exchange factor SEC2 (SEC2), partial

  • 中文名稱：
    釀酒酵母SEC2重組蛋白
  • 貨號：
    CSB-YP325476SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SEC2重組蛋白
  • 貨號：
    CSB-EP325476SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SEC2重組蛋白
  • 貨號：
    CSB-EP325476SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SEC2重組蛋白
  • 貨號：
    CSB-BP325476SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SEC2重組蛋白
  • 貨號：
    CSB-MP325476SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SEC2
  • Uniprot No.：
  • 別名：
    SEC2; YNL272C; N0641Rab guanine nucleotide exchange factor SEC2; GDP-GTP exchange factor SEC2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Guanine nucleotide exchange factor for SEC4, catalyzing the dissociation of GDP from SEC4 and also potently promoting binding of GTP. Activation of SEC4 by SEC2 is needed for the directed transport of vesicles to sites of exocytosis. Binds the Rab GTPase YPT32, but does not have exchange activity on YPT32.
  • 基因功能參考文獻：
    1. Yck1p and Yck2p phosphorylate sites within the Sec2p Ypt32p/Sec15p binding region and in doing so promote binding of Sec2p to Sec15p and inhibit binding to Ypt32p. PMID: 26700316
    2. Structural investigation of the Sec4p-Sec2p complex reveals the role of the Sec2p coiled-coil domain in facilitating the fast kinetics of the exchange reaction. PMID: 26263895
    3. The phosphorylation state of Sec2p can direct a switch in its regulatory binding partners that facilitates maturation of the secretory vesicle and helps to promote the directionality of vesicular transport. PMID: 24248333
    4. Sec2p is normally released from the exocyst after vesicle tethering so that it can recycle onto a new round of vesicles. PMID: 16611746
    5. The crystal structure of the Sec2p GEF domain at 3.0 A resolution was reported. PMID: 17292842
    6. A hypothetical model of the exchange domain of sec2p as a pair of coiled-coil helices that binds to sec4p and facilitates nucleotide disassociation. PMID: 17346229

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  • 亞細(xì)胞定位：
    Bud neck. Bud tip. Cytoplasmic vesicle, secretory vesicle. Note=Localizes to sites of polarized growth, namely to the bud neck and to the bud tip of growing buds, and associates with membranes. Colocalizes with secretory vesicles at exocytic sites. Proper localization is dependent on the actin cytoskeleton, MYO2, the kinesin-related protein SMY1, the Rab GTPase YPT32, and the production of post-Golgi vesicles.
  • 蛋白家族：
    SEC2 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YNL272C

    STRING: 4932.YNL272C



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