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  4. Recombinant Saccharomyces cerevisiae RNA polymerase II subunit B1 CTD phosphatase RTR1 (RTR1)

Recombinant Saccharomyces cerevisiae RNA polymerase II subunit B1 CTD phosphatase RTR1 (RTR1)

  • 中文名稱：
    釀酒酵母RTR1重組蛋白
  • 貨號：
    CSB-YP328070SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RTR1重組蛋白
  • 貨號：
    CSB-EP328070SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RTR1重組蛋白
  • 貨號：
    CSB-EP328070SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RTR1重組蛋白
  • 貨號：
    CSB-BP328070SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RTR1重組蛋白
  • 貨號：
    CSB-MP328070SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RTR1
  • Uniprot No.：
  • 別名：
    RTR1; YER139C; RNA polymerase II subunit B1 CTD phosphatase RTR1; EC 3.1.3.16; RNA polymerase II-associated protein 2 homolog RTR1; Regulator of transcription 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-226
  • 氨基酸序列
    MATIEDIKET ALIPFQKHRQ LSMHEAEVIT LEIIGLLCDS ECKDEKTLKY LGRFLTPDMY QDLVDERNLN KRCGYPLCGK SPERIRDPFS MNDTTKKFLL ENNPYAYLSH YCSKFHFRCS QFYQVQLSDE ALFARTGVHL FEDPEQDKHD IDFKVTLFEE LLREKASEED IKSLISGLKK LGLNPDSGTT EKDDTELEDD LSKWLAQIKI VENDNPSILG DFTRED
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    RNA polymerase II subunit B1 C-terminal domain (CTD) phosphatase that dephosphorylates 'Ser-5' of the CTD and regulates RNA polymerase II during the transition from 'Ser-5' to 'Ser-2' phosphorylation.
  • 基因功能參考文獻(xiàn)：
    1. Findings show that Rtr1 is a global regulator of the C-terminal domain (CTD) code with deletion of RTR1 causing genome-wide changes in Ser5-P CTD phosphorylation and cotranscriptional histone H3 lysine 36 trimethylation. PMID: 27247267
    2. Data indicate that the structure of the phosphoryl transfer domain and reaction mechanism for the phosphoryl transfer activity of regulator of transcription 1 (Rtr1) is distinct from those of other phosphatase families. PMID: 26933063
    3. Rtr1p may imprint its RNA targets cotranscriptionally and determine their downstream degradation mechanism by directing these transcripts to a novel turnover pathway that involves Rtr1p, Dhh1p, and the REX family of exonucleases. PMID: 26843527
    4. Studies revealed that hyperphosphorylated RNAPII is the primary interacting partner for Rtr1. PMID: 24671508
    5. YER139C encodes the closest yeast homolog of the human RPAP2 protein, recently identified as a novel RNA polymerase II (RNAPII)-associated factor. PMID: 18408053
    6. Rtr1 functions as a CTD phosphatase essential for the Serine5-to-Serine2-Phosphorylation transition. PMID: 19394294

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Note=Shuttles constitutively between the cytoplasm and the nucleus.
  • 蛋白家族：
    RPAP2 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YER139C

    STRING: 4932.YER139C



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