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Recombinant Saccharomyces cerevisiae Putative methyltransferase BUD23 (BUD23)

  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Putative methyltransferase BUD23(BUD23)
  • 貨號:
    CSB-YP326560SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Putative methyltransferase BUD23(BUD23)
  • 貨號:
    CSB-EP326560SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Putative methyltransferase BUD23(BUD23)
  • 貨號:
    CSB-EP326560SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Putative methyltransferase BUD23(BUD23)
  • 貨號:
    CSB-BP326560SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae Putative methyltransferase BUD23(BUD23)
  • 貨號:
    CSB-MP326560SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    BUD23
  • Uniprot No.:
  • 別名:
    BUD23; YCR047C; YCR47C18S rRNA; guanine(1575)-N(7))-methyltransferase; EC 2.1.1.309; Bud site selection protein 23
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-275
  • 氨基酸序列
    MSRPEELAPP EIFYNDSEAH KYTGSTRVQH IQAKMTLRAL ELLNLQPCSF ILDIGCGSGL SGEILTQEGD HVWCGLDISP SMLATGLSRE LEGDLMLQDM GTGIPFRAGS FDAAISISAI QWLCNADTSY NDPKQRLMRF FNTLYAALKK GGKFVAQFYP KNDDQVDDIL QSAKVAGFSG GLVVDDPESK KNKKYYLVLS SGAPPQGEEQ VNLDGVTMDE ENVNLKKQLR QRLKGGKDKE SAKSFILRKK ELMKRRGRKV AKDSKFTGRK RRHRF
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of guanine 1575 (m7G1575) in 18S rRNA. Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity. Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity. Required for efficient cleavage of the primary 35S precursor rRNA at site A2. Involved in positioning the proximal bud pole signal.
  • 基因功能參考文獻:
    1. BUD23 works at the transition of early to middle stage of 40S biogenesis. PMID: 23604635
    2. The absence of Bud23, Bud23 (G57R) at a high level might affect the cell division of yeast cells through an as yet unidentified mechanism. PMID: 23233232
    3. Mutations in Bud23 that inactivated its methyltransferase activity complemented a bud23Delta mutant. In addition, mutant ribosomes in which G1575 was changed to adenosine supported growth comparable to that of cells with wild-type ribosomes. PMID: 18332120
  • 亞細胞定位:
    Cytoplasm. Nucleus.
  • 蛋白家族:
    Class I-like SAM-binding methyltransferase superfamily, BUD23/WBSCR22 family
  • 數據庫鏈接:

    KEGG: sce:YCR047C

    STRING: 4932.YCR047C



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