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  4. Recombinant Saccharomyces cerevisiae Protein kinase C-like 1 (PKC1), partial

Recombinant Saccharomyces cerevisiae Protein kinase C-like 1 (PKC1), partial

  • 中文名稱：
    釀酒酵母PKC1重組蛋白
  • 貨號：
    CSB-YP326153SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母PKC1重組蛋白
  • 貨號：
    CSB-EP326153SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母PKC1重組蛋白
  • 貨號：
    CSB-EP326153SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母PKC1重組蛋白
  • 貨號：
    CSB-BP326153SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母PKC1重組蛋白
  • 貨號：
    CSB-MP326153SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PKC1
  • Uniprot No.：
  • 別名：
    PKC1; HPO2; STT1; YBL105C; YBL0807Protein kinase C-like 1; PKC 1; EC 2.7.11.13
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for cell growth and for the G2->M transition of the cell division cycle. Mediates a protein kinase cascade; it activates BCK1 which itself activates MKK1/MKK2.
  • 基因功能參考文獻：
    1. Pkc1 in cooperation with calcineurin mediates cell survival under compressive mechanical stress. PMID: 29196524
    2. The Pkc1-mediated degradation of Pah1 was attenuated in the cho1Delta mutant, which is deficient in PS synthase, supporting the notion that the phospholipid regulates Pkc1 activity in vivo. PMID: 28154205
    3. Constitutively active Pkc1 can drive cells through a mitotic checkpoint arrest, which suggests that Pkc1-dependent activation of PP2A(Cdc55) plays a critical role in checkpoint signaling. PMID: 28100785
    4. Pkc1 also enhances H3T45 phosphorylation and this modification is required for H3K56 acetylation. PMID: 26468616
    5. Authors propose that Rho1 and Pkc1 coordinate septin ring assembly with membrane and cell wall remodeling partly by controlling Syp1 residence at the bud neck. PMID: 26179915
    6. The Mih1 is negatively regulated by Pkc1-mediated phosphorylation, which excludes it from the nucleus under certain conditions. PMID: 23510481
    7. This study connects the Pkc1 protein to the maintenance of genome integrity in response to genotoxic stresses. PMID: 24792164
    8. Data indicate that Pkc1-induced phosphorylation of the eisosome core components Pil1 and Lsp1 was not exerted directly by Pkc1, but involved signaling through the Slt2 MAPK module. PMID: 23221999
    9. Pkc1p has important role in controlling the correct temporal expression of genes in the cell cycle of Saccharomyces cerevisiae. PMID: 22966207
    10. The Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. PMID: 22093750
    11. The authors demonstrate that Pfy1 promotes actin polymerization under conditions of oxidative stress, while Pkc1 induces actin polymerization and cell survival under all the conditions tested. PMID: 19765090
    12. Pkc1 activity is required to overcome the effects of oxidative stress. PMID: 15637049
    13. Pkc1p may have a role in regulating microtubule function PMID: 15643058
    14. The PKC1 protein is overexpression in Saccharomyces cerevisiae PMID: 15664515
    15. Under heat stress conditions activation of PKC1 pathway is triggered by an increase in intracellular osmolarity, due to the accumulation of trehalose, rather than by the increase in temperature as such. PMID: 16085846
    16. Pkc1p function was required for posttranscriptional upregulation of Cln2p and appeared to be important for the coordinated regulation of polar bud growth and the cell cycle. PMID: 16141237
    17. Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant. PMID: 16157662
    18. Ubp3 can regulate Pkc1 by facilitating its destruction and provide the initial evidence that Pkc1 plays a positive role in modulating the parallel pheromone-signaling pathway. PMID: 17986446
    19. KNR4 is essential for cell viability in the absence of a functional Pkc1-Slt2 pathway, since the lethality caused by KNR4 deletion in such a genetic background could not be compensated by overexpression of any gene from yeast genomic libraries PMID: 18668512

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  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
  • 數據庫鏈接：

    KEGG: sce:YBL105C

    STRING: 4932.YBL105C



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