Recombinant Saccharomyces cerevisiae Protein SIS1 (SIS1)

中文名稱：

釀酒酵母SIS1重組蛋白
貨號：

CSB-YP326198SVG
規格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母SIS1重組蛋白
貨號：

CSB-EP326198SVG
規格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母SIS1重組蛋白
貨號：

CSB-EP326198SVG-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母SIS1重組蛋白
貨號：

CSB-BP326198SVG
規格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母SIS1重組蛋白
貨號：

CSB-MP326198SVG
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SIS1
Uniprot No.：

P25294
別名：

SIS1; YNL007C; N2879; Protein SIS1
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

full length protein
表達區域：

1-352
氨基酸序列

MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE AFEILNDPQK REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF SGGHAFSNED AFNIFSQFFG GSSPFGGADD SGFSFSSYPS GGGAGMGGMP GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE TVQVNLPVSL EDLFVGKKKS FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN PQTGRRKTLQ FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE NF
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Required for nuclear migration during mitosis. It is required for the normal initiation of translation. Might mediate the dissociation of a specific protein complex of the translation machinery. Essential for viability.
基因功能參考文獻：
1. Differences in sensitivity of prion fragmentation to Sis1 and in relative inputs of fragmentation and malpartition in prion propagation result in opposite effects of Sis1 relocalization on [PSI (+)] and [URE3]. PMID: 28932898
2. Sis1 plays a role in prion replication. PMID: 25329162
3. Interaction of Sis1 with EEVD(Hsp70) minimizes transfer of Sis1-bound clients to Hsp70s that are primed for client transfer to folding and translocation pathways by their preassociation with EEVD binding adaptor proteins. PMID: 25687964
4. This conservation of function is also prion-variant dependent, indicating that only one of the two Sis1-prion functions may have been maintained in eukaryotic chaperone evolution. PMID: 25058638
5. The stability of Sis1 and mutants that had the central domains (named Gly/Met rich domain and C-terminal Domain I) deleted or switched to those of Ydj1 were evaluated to gain insight into the role of these regions in the structure and function of Sis1. PMID: 23227221
6. Sis1 is a yeast member of the Type II Hsp40s family and in a highly conserved alpha-helical N-terminal J-domain, a disordered middle region (divided into glycine/phenylalanine (G/F) and glycine/methionine (G/M) rich regions) and two C-terminal sub-domains. PMID: 22011374
7. Functions of yeast Hsp40 chaperone Sis1p are dispensable for prion propagation but important for prion curing and protection from prion toxicity. PMID: 21555396
8. Genetic experiments showed that Sis1 is an essential protein and apparently plays a functional role at assembly or disassembly of protein complexes. PMID: 17985367
9. In vivo monitoring of the prion replication cycle reveals a critical role for Sis1 in delivering substrates to Hsp104. PMID: 19026788
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亞細胞定位：

Cytoplasm. Nucleus. Note=Localized throughout the cell but is more concentrated at the nucleus.
數據庫鏈接：

KEGG: sce:YNL007C

STRING: 4932.YNL007C