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  4. Recombinant Saccharomyces cerevisiae Protein SFI1 (SFI1), partial

Recombinant Saccharomyces cerevisiae Protein SFI1 (SFI1), partial

  • 中文名稱：
    釀酒酵母SFI1重組蛋白
  • 貨號：
    CSB-YP619590SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SFI1重組蛋白
  • 貨號：
    CSB-EP619590SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SFI1重組蛋白
  • 貨號：
    CSB-EP619590SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SFI1重組蛋白
  • 貨號：
    CSB-BP619590SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SFI1重組蛋白
  • 貨號：
    CSB-MP619590SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SFI1
  • Uniprot No.：
  • 別名：
    SFI1; YLL003W; L1373; Protein SFI1; Suppressor of fermentation induced loss of stress resistance protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of the spindle pole body (SPB) half-bridge involved in the initial steps of SPB duplication.
  • 基因功能參考文獻：
    1. Kar1 binding is restricted to Sfi1-C-terminal and Sfi1 C-terminal centrin-binding repeats, and centrin and Kar1 provide cross-links, while Sfi1-C-terminal stabilizes the bridge and ensures timely spindle pole body separation. PMID: 26076691
    2. Our findings lead to a model whereby phosphoregulation of Sfi1 by Cdk1 has the dual function of promoting spindle pole bodies separation for spindle formation and preventing premature SPB duplication. PMID: 25340401
    3. After spindle pole body duplication, the dimerized Sfi1 is severed to allow spindle formation and spindle pole body reduplication. PMID: 24954044
    4. Sfi1 bound with Cdc31 in a complex have a role in spindle pole body duplication. PMID: 16785321
    5. Results describe additional functions for Sfi1p in bipolar spindle assembly and function based on the actions of several novel sfi1 alleles. PMID: 17392514

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    SFI1 family
  • 數據庫鏈接：

    KEGG: sce:YLL003W

    STRING: 4932.YLL003W



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