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  4. Recombinant Saccharomyces cerevisiae Protein DOM34 (DOM34)

Recombinant Saccharomyces cerevisiae Protein DOM34 (DOM34)

  • 中文名稱：
    釀酒酵母DOM34重組蛋白
  • 貨號：
    CSB-YP335817SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母DOM34重組蛋白
  • 貨號：
    CSB-EP335817SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母DOM34重組蛋白
  • 貨號：
    CSB-EP335817SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母DOM34重組蛋白
  • 貨號：
    CSB-BP335817SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母DOM34重組蛋白
  • 貨號：
    CSB-MP335817SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DOM34
  • Uniprot No.：
  • 別名：
    DOM34; YNL001W; N2016; Protein DOM34; EC 3.1.-.-
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-386
  • 氨基酸序列
    MKVISLKKDS FNKGGAVITL LPEDKEDLFT VYQIVDKDDE LIFKKKFTSK LDEAGKKKST DLVKLKIKVI SEDFDMKDEY LKYKGVTVTD ESGASNVDIP VGKYLSFTLD YVYPFTIIKQ NFNKFMQKLL NEACNIEYKS DTAAVVLQEG IAHVCLVTSS STILKQKIEY SMPKKKRTTD VLKFDEKTEK FYKAIYSAMK KDLNFDKLKT IILCSPGFYA KILMDKIFQY AEEEHNKKIL DNKGMFFIAH CSTGYLQGIN EVLKNPLYAS KLQDTKYSKE IMVMDEFLLH LNKDDDKAWY GEKEVVKAAE YGAISYLLLT DKVLHSDNIA QREEYLKLMD SVESNGGKAL VLSTLHSLGE ELDQLTGIAC ILKYPLPDLD EDDGEE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in protein translation. Together with HBS1, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. The complex formed by DOM34 and HBS1 has ribonuclease activity towards double-stranded RNA substrates, but does not cleave single-stranded RNA. Acts as endonuclease; has no exonuclease activity. Increases the affinity of HBS1 for GTP, but nor for GDP. Promotes G1 progression and differentiation and is involved in mitotic and meiotic cell divisions.
  • 基因功能參考文獻：
    1. Study found that Dom34 rescues ribosomes that stall at the 3' end of mRNAs that are truncated. Dom34 rescues ribosomes in the 3' untranslated region of many genes. Surprisingly, many ribosomes populate this noncoding region, and these ribosomes seem to move along the mRNA without recognizing codons, presumably not translating, and eventually arrest. PMID: 24581494
    2. The slow growth of cells lacking Dom34 and haploinsufficient for a protein of the 40S subunit is caused by a severe shortage of 40S subunits available for translation initiation. PMID: 20876302
    3. Results identify important functional regions of Dom34 and suggest that the proposed endonuclease activity of Dom34 is not required for mRNA cleavage in NGD. PMID: 19420139
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Eukaryotic release factor 1 family, Pelota subfamily
  • 數據庫鏈接：

    KEGG: sce:YNL001W

    STRING: 4932.YNL001W



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