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  4. Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2 (BRR2), partial

Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2 (BRR2), partial

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2(BRR2) ,partial
  • 貨號：
    CSB-YP327153SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2(BRR2) ,partial
  • 貨號：
    CSB-EP327153SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2(BRR2) ,partial
  • 貨號：
    CSB-EP327153SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2(BRR2) ,partial
  • 貨號：
    CSB-BP327153SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing helicase BRR2(BRR2) ,partial
  • 貨號：
    CSB-MP327153SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    BRR2
  • Uniprot No.：
  • 別名：
    BRR2; RSS1; SNU246; YER172C; SYGP-ORF66Pre-mRNA-splicing helicase BRR2; EC 3.6.4.13; Protein Snu246
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.
  • 基因功能參考文獻：
    1. Data show that the truncation of the first 247 amino acids of the N terminus in Bad Response to Refrigeration 2 (Delta247-Brr2) weakened RNA binding, reduced helicase activity, and reduced ATPase activity compared with wild type Brr2. PMID: 27072132
    2. the N-terminal region autoinhibits Brr2 via substrate competition and conformational clamping PMID: 26637280
    3. The N-terminal domain iscritical for retaining U5 and U6 snRNPs during/after spliceosomal activation through its interaction with snRNAs and other spliceosomal proteins, revealing a role of Brr2 in spliceosomal activation in addition to U4/U6 unwinding. PMID: 25670679
    4. structure explains why Aar2 and Brr2 are mutually exclusive and provides important insights into the assembly of U5 snRNP PMID: 23727230
    5. function for Brr2p in driving conformational rearrangements that lead to competence for the second step of splicing PMID: 23124065
    6. the mechanism whereby Brr2 unwinds U4/U6 and show how this activity is potentially regulated prior to spliceosome activation PMID: 23124066
    7. Results suggest that Brr2 shares a similar helicase mechanism with Hel308, and demonstrate that Hel308-II interacts with Prp8 and Snu114 in vitro and in vivo. PMID: 19525970

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Helicase family, SKI2 subfamily
  • 數據庫鏈接：

    KEGG: sce:YER172C

    STRING: 4932.YER172C



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