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  4. Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor CWC2 (CWC2)

Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor CWC2 (CWC2)

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor CWC2(CWC2)
  • 貨號：
    CSB-YP612274SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor CWC2(CWC2)
  • 貨號：
    CSB-EP612274SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor CWC2(CWC2)
  • 貨號：
    CSB-EP612274SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor CWC2(CWC2)
  • 貨號：
    CSB-BP612274SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor CWC2(CWC2)
  • 貨號：
    CSB-MP612274SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CWC2
  • Uniprot No.：
  • 別名：
    CWC2; NTC40; SLC3; YDL209C; D1041Pre-mRNA-splicing factor CWC2; Complexed with CEF1 protein 2; PRP19-associated complex protein 40; Synthetic lethal with CLF1 protein 3
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-339
  • 氨基酸序列
    MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEYL HHIPDEEDIG KLALRTEVLD CFGREKFADY REDMGGIGSF RKKNKTLYVG GIDGALNSKH LKPAQIESRI RFVFSRLGDI DRIRYVESKN CGFVKFKYQA NAEFAKEAMS NQTLLLPSDK EWDDRREGTG LLVKWANEDP DPAAQKRLQE ELKLESLNMM VHLINNNTNS AGTEVNNKNN ERLDRTFPEA SVDNVKKRLL PLDNGMESDD FIEKLKKVKK NISRENISSK PSVGKLGGPL LDYLSSDED
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the 'nineteen complex' NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1, U4, U5 and U6 snRNAs and to pre-mRNAs, in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.
  • 基因功能參考文獻：
    1. The study found that one function of Cwc2 is to stabilize U2-U6 snRNA helix I during splicing. PMID: 24848011
    2. The S. cerevisiae Cwc2 protein functions prior to step 1 of splicing. PMID: 22246180
    3. we report the 1.9 A (1 A=0.1 nm) X-ray structure of the Cwc2 core domain, which is both necessary and sufficient for RNA binding. PMID: 21957909
    4. Yeast cells depleted of Cwc2 accumulate pre-mRNA and display reduced levels of U1, U4, U5 and U6 snRNAs. PMID: 19435883
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    RRM CWC2 family
  • 數據庫鏈接：

    KEGG: sce:YDL209C

    STRING: 4932.YDL209C



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