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  4. Recombinant Saccharomyces cerevisiae Poly (A) RNA polymerase protein 2 (PAP2), partial

Recombinant Saccharomyces cerevisiae Poly (A) RNA polymerase protein 2 (PAP2), partial

  • 中文名稱：
    釀酒酵母PAP2重組蛋白
  • 貨號：
    CSB-YP345695SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母PAP2重組蛋白
  • 貨號：
    CSB-EP345695SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母PAP2重組蛋白
  • 貨號：
    CSB-EP345695SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母PAP2重組蛋白
  • 貨號：
    CSB-BP345695SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母PAP2重組蛋白
  • 貨號：
    CSB-MP345695SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PAP2
  • Uniprot No.：
  • 別名：
    PAP2; TRF4; YOL115W; HRC584; O0716; Poly(A) RNA polymerase protein 2; EC 2.7.7.19; DNA polymerase kappa; DNA polymerase sigma; Topoisomerase 1-related protein TRF4
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker\'s yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalytic subunit of the TRAMP complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates like cryptic transcripts generated by RNA polymerase II and III, or hypomethylated pre-tRNAi-Met. Polyadenylates RNA processing and degradation intermediates of snRNAs, snoRNAs and mRNAs that accumulate in strains lacking a functional exosome. TRF4 is also required for proper nuclear division in mitosis, DNA damage repair and sister chromatid cohesion. Involved in the regulation of histone mRNA levels. May mediate mitotic chromosome condensation.
  • 基因功能參考文獻：
    1. Results show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro. PMID: 25066235
    2. Trf4p is the catalytic subunit of a new poly(A) polymerase complex that contains Air1p or Air2p as potential RNA-binding subunits. PMID: 15828860
    3. Trf4 and Trf5 protein complexes have essential roles in generating functional mRNA poly(A) tails in the nucleus PMID: 16260630
    4. RNA processing and degradation intermediates are polyadenylated, and that their polyadenylation is dependent on the activity of Trf4p and Trf5p, two variant poly(A) polymerases. PMID: 16373491
    5. a catalytically active Trf4p poly(A) polymerase is required for polyadenylation of hypomethylated tRNAi(Met) in vivo PMID: 16431988
    6. TRF4, TRF5, and RRP6 play a role in the regulation of histone mRNA levels in yeast. PMID: 17179095
    7. Trf4 protein has intrinsic 5'-deoxyribose-5-phosphate lyase activity and a possible role in base excision DNA repair. PMID: 17983848
    8. Results report that in Saccharomyces cerevisiae strains lacking Trf4, cryptic transcripts are detected from regions of repressed chromatin at telomeres and the rDNA intergenic spacer region (IGS1-R), and at CEN3. PMID: 18007593

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-B-like family
  • 數據庫鏈接：

    KEGG: sce:YOL115W

    STRING: 4932.YOL115W



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