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  4. Recombinant Saccharomyces cerevisiae Phosphate system positive regulatory protein PHO4 (PHO4)

Recombinant Saccharomyces cerevisiae Phosphate system positive regulatory protein PHO4 (PHO4)

  • 中文名稱：
    釀酒酵母PHO4重組蛋白
  • 貨號：
    CSB-YP357073SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母PHO4重組蛋白
  • 貨號：
    CSB-EP357073SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母PHO4重組蛋白
  • 貨號：
    CSB-EP357073SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母PHO4重組蛋白
  • 貨號：
    CSB-BP357073SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母PHO4重組蛋白
  • 貨號：
    CSB-MP357073SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PHO4
  • Uniprot No.：
  • 別名：
    PHO4; YFR034C; Phosphate system positive regulatory protein PHO4
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-312
  • 氨基酸序列
    MGRTTSEGIH GFVDDLEPKS SILDKVGDFI TVNTKRHDGR EDFNEQNDEL NSQENHNSSE NGNENENEQD SLALDDLDRA FELVEGMDMD WMMPSHAHHS PATTATIKPR LLYSPLIHTQ SAVPVTISPN LVATATSTTS ANKVTKNKSN SSPYLNKRRG KPGPDSATSL FELPDSVIPT PKPKPKPKQY PKVILPSNST RRVSPVTAKT SSSAEGVVVA SESPVIAPHG SSHSRSLSKR RSSGALVDDD KRESHKHAEQ ARRNRLAVAL HELASLIPAE WKQQNVSAAP SKATTVEAAC RYIRHLQQNV ST
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcriptional activator that regulates the expression of repressible phosphatase under phosphate starvation conditions. Binds to the upstream activating sequence (UAS) of several phosphatase encoding PHO genes. Inhibited by the cyclin-CDK PHO80-PHO85 under high-phosphate conditions.
  • 基因功能參考文獻：
    1. Pho85 and Pho4 regulated CLN2 expression under alkaline condition. PMID: 25582350
    2. Subsequent in-depth analysis of the inducible PHO5 gene reveals that topoisomerases are essential for binding of the Pho4p transcription factor to the PHO5 promoter, which is required for promoter nucleosome removal during activation. PMID: 23284296
    3. The authors postulate that RPL12 has an extra-ribosomal function in modulating the transcription of genes that need Pho4p activation. PMID: 21469193
    4. three transcriptional outputs can be generated by a pathway whose regulation is controlled by one kinase, Pho80-Pho85, and one transcription factor, Pho4 PMID: 14624238
    5. These data establish a G1 checkpoint adaptation pathway where Pho85 mediates Pho4 downregulation and Sic1 degradation to release Cdc28 activity and promote onset of S phase. PMID: 16964260
    6. Pho4 binding to the SNZ1/SNO1 promoter is accompanied by alterations in chromatin structure, and Rpd3 histone deacetylase is required for the proper timing of SNZ1/SNO1 expression, while Asf1 histone chaperone is indispensable for their expression. PMID: 18408055
    7. Pho4 activates some stress response genes, is required for G1 arrest caused by DNA damage, and is present in the nucleus even under high-Pi conditions to activate or repress transcription. PMID: 19108609

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Note=Predominantly cytoplasmic under high-phosphate conditions and localized to the nucleus upon phosphate starvation.
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YFR034C

    STRING: 4932.YFR034C



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