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  4. Recombinant Saccharomyces cerevisiae Pachytene checkpoint protein 2 (PCH2), partial

Recombinant Saccharomyces cerevisiae Pachytene checkpoint protein 2 (PCH2), partial

  • 中文名稱：
    釀酒酵母PCH2重組蛋白
  • 貨號：
    CSB-YP339918SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母PCH2重組蛋白
  • 貨號：
    CSB-EP339918SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母PCH2重組蛋白
  • 貨號：
    CSB-EP339918SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母PCH2重組蛋白
  • 貨號：
    CSB-BP339918SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母PCH2重組蛋白
  • 貨號：
    CSB-MP339918SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PCH2
  • Uniprot No.：
  • 別名：
    PCH2; YBR186W; YBR1308; Pachytene checkpoint protein 2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for the pachytene checkpoint, the meiotic checkpoint that prevents chromosome segregation when defects in recombination and synaptonemal complex formation occurred. Represses meiotic recombination in the rDNA, probably by excluding the meiosis-specific protein HOP1 from the nucleolar region.
  • 基因功能參考文獻：
    1. pch2Delta showed approximately 50% genome-wide increase in crossover frequency, elevated noncrossovers and loss of chromosome size dependent double-strand break formation. PMID: 28315832
    2. discuss our current understanding of the function of Pch2(TRIP13) during meiotic and mitotic cell divisions, with a focus on its enzymatic role towards HORMA domain-containing clients PMID: 25895724
    3. Pch2 displaces Hop1 from large DNA substrates and both ATP binding and hydrolysis by Pch2 are required for Pch2-Hop1 transactions PMID: 24367111
    4. Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae. PMID: 21515575
    5. tgs1Delta cells are specifically defective in splicing PCH2 meiotic pre-mRNAs. PMID: 21398639
    6. PCH2 is required for recombination and normal higher-order chromosome structure during meiosis. PMID: 20711356
    7. during WT meiosis, Pch2 promotes progression of SC-associated CO and NCO recombination complexes at a regulated early-midpachytene transition that is rate-limiting for later events PMID: 18305165
    8. Roles of Pch2 in controlling both chromosome axis morphogenesis and crossover placement suggest linkage between these processes. PMID: 19629172
    9. Study provides four evidence suggesting that PCH2 regulates the formation and distribution of crossover events required to promote chromosome segregation at Meiosis I. PMID: 19629178

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  • 亞細胞定位：
    Nucleus, nucleolus. Chromosome. Note=The majority of the protein localizes to the nucleolar region containing the ribosomal RNA genes (rDNA), a tandem array of 100-150 repeats on chromosome XII. Localization is SIR2-dependent.
  • 蛋白家族：
    AAA ATPase family, PCH2 subfamily
  • 數據庫鏈接：

    KEGG: sce:YBR186W

    STRING: 4932.YBR186W



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