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  4. Recombinant Saccharomyces cerevisiae PAB-dependent poly (A)-specific ribonuclease subunit PAN2, partial

Recombinant Saccharomyces cerevisiae PAB-dependent poly (A)-specific ribonuclease subunit PAN2, partial

  • 中文名稱：
    釀酒酵母PAN2重組蛋白
  • 貨號：
    CSB-YP346035SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母PAN2重組蛋白
  • 貨號：
    CSB-EP346035SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母PAN2重組蛋白
  • 貨號：
    CSB-EP346035SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母PAN2重組蛋白
  • 貨號：
    CSB-BP346035SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母PAN2重組蛋白
  • 貨號：
    CSB-MP346035SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    PAN2; YGL094C; PAN2-PAN3 deadenylation complex catalytic subunit PAN2; EC 3.1.13.4; PAB1P-dependent poly(A)-specific ribonuclease; Poly(A)-nuclease deadenylation complex subunit 2; PAN deadenylation complex subunit 2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping by DCP1-DCP2 and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails, trimming the tails from their synthesized length to the slightly shorter, apparently messenger-specific length found on newly exported mRNAs. PAN cooperates with protein kinase DUN1 in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress.
  • 基因功能參考文獻：
    1. PAN2 and PAN3 are not required for mRNA poly(A) tail length control during de novo synthesis but may act in conjuction with Pab1p at a later stage in mRNA metabolism. PMID: 15894541
    2. The exonuclease subunit Pan2 does not bind RNA by itself, but binds to an asymmetric Pan3 homodimer via an extensive, high-affinity interaction. PMID: 24872509
    3. Accumulation of polyadenylated mRNAs at sites of transcription occurs in strains lacking the PAN2 or PAN3 subunits of the poly(A) nuclease PAN. PAN is identified as an important factor that couples 3' processing of pre-mRNAs to export. PMID: 15630021
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C19 family, PAN2 subfamily
  • 數據庫鏈接：

    KEGG: sce:YGL094C

    STRING: 4932.YGL094C



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