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  2. 蛋白
  3. 重組蛋白
  4. Recombinant Saccharomyces cerevisiae Nucleolar protein 3 (NPL3)

Recombinant Saccharomyces cerevisiae Nucleolar protein 3 (NPL3)

  • 中文名稱：
    釀酒酵母NPL3重組蛋白
  • 貨號(hào)：
    CSB-YP015921SVG
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母NPL3重組蛋白
  • 貨號(hào)：
    CSB-EP015921SVG
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母NPL3重組蛋白
  • 貨號(hào)：
    CSB-EP015921SVG-B
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母NPL3重組蛋白
  • 貨號(hào)：
    CSB-BP015921SVG
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母NPL3重組蛋白
  • 貨號(hào)：
    CSB-MP015921SVG
  • 說(shuō)明書：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NPL3
  • Uniprot No.：
  • 別名：
    NPL3; MTR13; MTS1; NAB1; NOP3; YDR432W; D9461.19; Nucleolar protein 3; Mitochondrial targeting suppressor 1 protein; Nuclear polyadenylated RNA-binding protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-414
  • 氨基酸序列
    MSEAQETHVE QLPESVVDAP VEEQHQEPPQ APDAPQEPQV PQESAPQESA PQEPPAPQEQ NDVPPPSNAP IYEGEESHSV QDYQEAHQHH QPPEPQPYYP PPPPGEHMHG RPPMHHRQEG ELSNTRLFVR PFPLDVQESE LNEIFGPFGP MKEVKILNGF AFVEFEEAES AAKAIEEVHG KSFANQPLEV VYSKLPAKRY RITMKNLPEG CSWQDLKDLA RENSLETTFS SVNTRDFDGT GALEFPSEEI LVEALERLNN IEFRGSVITV ERDDNPPPIR RSNRGGFRGR GGFRGGFRGG FRGGFSRGGF GGPRGGFGGP RGGYGGYSRG GYGGYSRGGY GGSRGGYDSP RGGYDSPRGG YSRGGYGGPR NDYGPPRGSY GGSRGGYDGP RGDYGPPRDA YRTRDAPRER SPTR
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for pre-rRNA processing and nuclear import as well as mitochondrial protein targeting. Binds to poly(A).
  • 基因功能參考文獻(xiàn)：
    1. Npl3 participates in DNA double-strand break resection by promoting the proper biogenesis of EXO1 mRNA. PMID: 28472517
    2. a model in which Hmt1, via arginine methylation of Npl3, facilitates U1 snRNP engagement with the pre-mRNA to promote usage of non-consensus splice sites by the splicing machinery. PMID: 28392442
    3. Npl3 binds diverse sites on large numbers of transcripts, and the loss of Npl3 results in transcriptional readthrough on many genes. PMID: 26694144
    4. An essential novel function for Npl3 in the cytoplasm, which supports monosome formation for translation initiation. PMID: 24100011
    5. yeast Npl3, the most abundant RNA-binding hnRNP, prevents R-loop-mediated genome instability PMID: 24240235
    6. our data reveal a connection between Npl3 and an extensive array of chromatin factors and describe an unanticipated functional link between histone H2B ubiquitination and pre-mRNA splicing PMID: 23209445
    7. Npl3 and Cbc2 may function in maintaining telomere stability perhaps as telomere capping proteins. PMID: 22902537
    8. These results support a model in which arginine methylation facilitates Npl3 export directly by weakening contacts with nuclear proteins. PMID: 15998636
    9. NPL1p can bind to nascent mRNAs, heat shock RNA was exported efficiently in temperature-sensitive NPl13 mutant strains PMID: 17259545
    10. The results support a model in which balanced competition of Npl3 with mRNA processing factors may promote recognition of proper polyadenylation sites while suppressing cryptic sites. PMID: 17684230
    11. Improved segmental isotope labeling methods for the NMR study of multidomain of of large proteins: application to the RRMs of NPL3. PMID: 17936301
    12. Structure of NPL3 protein and its interaction with mRNA 3'-end processing signals is reported. PMID: 18022637
    13. Proper levels of Npl3 protein are achieved by a negative feedback loop in which phosphorylated Npl3 suppresses efficient recognition of the productive processing signals in its own transcript. PMID: 18391019
    14. Npl3 has a role in RNA polymerase II elongation and its regulation by phosphorylation PMID: 18818768
    15. A single SR-like protein, Npl3, is required for efficient splicing of a large set of pre-mRNAs in Saccharomyces cerevisiae PMID: 19061647
    16. Nuclear localization of Npl3 also requires specific arginine-glycine (RG) sequences, yet heterologous RG domains allow similar modulation of Npl3 transport by arginine methylation. PMID: 19454603
    17. model in which Npl3p engenders translational fidelity by promoting the remodeling of mRNPs during translation termination PMID: 19733178

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  • 亞細(xì)胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    RRM GAR family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YDR432W

    STRING: 4932.YDR432W



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