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  4. Recombinant Saccharomyces cerevisiae Non-histone chromosomal protein 6A (NHP6A)

Recombinant Saccharomyces cerevisiae Non-histone chromosomal protein 6A (NHP6A)

  • 中文名稱：
    釀酒酵母NHP6A重組蛋白
  • 貨號：
    CSB-YP320019SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母NHP6A重組蛋白
  • 貨號：
    CSB-EP320019SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母NHP6A重組蛋白
  • 貨號：
    CSB-EP320019SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母NHP6A重組蛋白
  • 貨號：
    CSB-BP320019SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母NHP6A重組蛋白
  • 貨號：
    CSB-MP320019SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NHP6A
  • Uniprot No.：
  • 別名：
    NHP6A; NHPA; YPR052C; YP9499.09C; Non-histone chromosomal protein 6A
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-93
  • 氨基酸序列
    MVTPREPKKR TTRKKKDPNA PKRALSAYMF FANENRDIVR SENPDITFGQ VGKKLGEKWK ALTPEEKQPY EAKAQADKKR YESEKELYNA TLA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6B.
  • 基因功能參考文獻：
    1. The yeast HMG box protein NHP6A shows DNA-segment-facilitated dissociation effects similar to those of Fis. PMID: 26220077
    2. adapt two high-resolution in vivo protein-mapping techniques to demonstrate direct binding of the heterologous Nhp6A protein at a LacI repression loop in living E. coli cells PMID: 26039992
    3. Observations support a model in which the HMGB protein Nhp6A soften DNA through random dynamic binding and dissociation, accompanied by DNA kinking and straightening, respectively. PMID: 23221634
    4. While all of the HMGB proteins bend DNA to preferred angles, Nhp6A promoted the formation of higher-order oligomer structures. PMID: 22197373
    5. Chromatin-dependent binding of the S. cerevisiae HMGB protein Nhp6A affects nucleosome dynamics and transcription PMID: 20844014
    6. Analysis of mechanical response generated by binding of DNA-bending protein NHP6A to single tethered 48.5 kb lambda-DNA molecules finds that compaction of DNA increases with increasing NHP6A concentration. PMID: 15504049
    7. NHP6A is required for transcription of a subset of tRNA genes and heterochromatin barrier function. PMID: 17178828
    8. Nhp6Ap has a role in the evolutionarily conserved calmodulin-dependent nuclear import pathway PMID: 17878171
    9. An isolated HMG box A domain from human HMGB2 does not enhance DNA flexibility. Substitution of a small number of cationic residues from the N-terminal leader of a functional yeast box B protein, Nhp6Ap, confers the ability to enhance DNA flexibility. PMID: 19236006

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  • 亞細胞定位：
    Nucleus. Chromosome. Note=Colocalizes with both RNA polymerase II and some regions that are not transcribed on chromatin.
  • 蛋白家族：
    NHP6 family
  • 數據庫鏈接：

    KEGG: sce:YPR052C

    STRING: 4932.YPR052C



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