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  4. Recombinant Saccharomyces cerevisiae Mitochondrial tRNA-specific 2-thiouridylase 1 (SLM3)

Recombinant Saccharomyces cerevisiae Mitochondrial tRNA-specific 2-thiouridylase 1 (SLM3)

  • 中文名稱：
    釀酒酵母SLM3重組蛋白
  • 貨號：
    CSB-YP604905SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SLM3重組蛋白
  • 貨號：
    CSB-EP604905SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SLM3重組蛋白
  • 貨號：
    CSB-EP604905SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SLM3重組蛋白
  • 貨號：
    CSB-BP604905SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SLM3重組蛋白
  • 貨號：
    CSB-MP604905SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SLM3
  • Uniprot No.：
  • 別名：
    SLM3; MTO2; MTU1; YDL033C; D2761; Mitochondrial tRNA-specific 2-thiouridylase 1; EC 2.8.1.14; Mitochondrial translation optimization protein 2; Synthetic lethal with MSS4 3
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-417
  • 氨基酸序列
    MLARYLNLIG RRSASPYRPQ RLPAKFDNVI VAMSSGVDSS VAAALFAGEF PNTRGVYMQN WSESQSLDDP GKEPCYERDW RDVNRVAKHL NIRVDKVNFE QDYWIDVFEP MLRGYSEGST PNPDIGCNKF VKFGKLREWL DEKYGTGNYW LVTGHYARVM QEMNGKGLFH LLRSIYRPKD QSYYLSQINS TVLSSLLLPI GHLTKPEVRD LAKYAGLPTA EKPDSQGICF VNNSQHGKFK NFLKHYLPSS PGDIITVDPQ SGAKTTWGRH DGLWSYTIGQ KVGISMPQAD PNYQGTWFVS EKLRDTNEIL IVRGRDNPAL YSDTMRIENF SSLGPREDTI NAFQNTGALT LQFRSLQVPV QIKSCKLNRS ADNLDITIHL ASKQRAITPG QSCCLYIDDR VLGSGPISHV NNNDTHA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.
  • 基因功能參考文獻：
    1. The identification and characterization of MTU1 (mitochondrial tRNA-specific 2-thiouridylase 1), a tRNA-modifying enzyme that is responsible for the 2-thiolation of the wobble position in mitochondrial tRNAs. [MTU1] PMID: 15509579
    2. These data strongly indicate that unmodified tRNA caused by the deletion of MTO2 caused the instability of mitochondrial tRNAs and mRNAs and impairment of aminoacylation of tRNAs. PMID: 17706197
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    MnmA/TRMU family
  • 數據庫鏈接：

    KEGG: sce:YDL033C

    STRING: 4932.YDL033C



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