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  4. Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM22 (TIM22), partial

Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM22 (TIM22), partial

  • 中文名稱：
    釀酒酵母TIM22重組蛋白
  • 貨號：
    CSB-YP615492SVG1
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母TIM22重組蛋白
  • 貨號：
    CSB-EP615492SVG1
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母TIM22重組蛋白
  • 貨號：
    CSB-EP615492SVG1-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母TIM22重組蛋白
  • 貨號：
    CSB-BP615492SVG1
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母TIM22重組蛋白
  • 貨號：
    CSB-MP615492SVG1
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TIM22
  • Uniprot No.：
  • 別名：
    TIM22; YDL217C; D0884; Mitochondrial import inner membrane translocase subunit TIM22
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins, such as mitochondrial carrier family members, into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps. Mediates the insertion of precursor proteins in a 3 step process. After the precursor is tethered to the translocase without losing energy from the Delta(psi), 2 energy-requiring steps are needed. First, Delta(psi) acts on the precursor protein and promotes its docking in the translocase complex. Then, Delta(psi) and an internal signal peptide together induce rapid gating transitions in one pore and closing of the other pore and drive membrane insertion to completion.
  • 基因功能參考文獻：
    1. reduced phosphatidylcholine levels differentially affect the TIM22 and TIM23 complexes in mitochondrial protein transport. PMID: 27402832
    2. We propose that Mia40 not only is responsible for disulfide bond formation, but also assists the Tim22 protein in its integration into the inner membrane of mitochondria. PMID: 23283984
    3. When Hot13p is overexpressed, it may function to remodel the small TIM complexes during import. PMID: 15294910
    4. the carboxyl-terminal module of dicarboxylate carrier possesses major targeting information to facilitate the direct binding of the carrier protein to TIM22 PMID: 15591051
    5. Phosphate carrier (PiC) can be sorted to either the TIM22 pathway or the TIM23 pathway for its import into yeast mitochondria PMID: 15644337
    6. The Tim21 structure represents a new alpha/beta-mixed protein fold with two alpha-helices flanked by an extended eight-stranded beta-sheet. Here we suggest a mechanism for the TOM-TIM interaction. PMID: 17099692
    7. TIM22 as a dynamic channel solely active in the presence of its cargo proteins PMID: 17462993

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  • 亞細胞定位：
    Mitochondrion inner membrane; Multi-pass membrane protein. Note=Import into inner membrane protein requires TOM20 function.
  • 蛋白家族：
    Tim17/Tim22/Tim23 family
  • 數據庫鏈接：

    KEGG: sce:YDL217C

    STRING: 4932.YDL217C



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