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  4. Recombinant Saccharomyces cerevisiae Meiosis-specific serine/threonine-protein kinase MEK1 (MEK1)

Recombinant Saccharomyces cerevisiae Meiosis-specific serine/threonine-protein kinase MEK1 (MEK1)

  • 中文名稱：
    釀酒酵母MEK1重組蛋白
  • 貨號：
    CSB-YP333015SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MEK1重組蛋白
  • 貨號：
    CSB-EP333015SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MEK1重組蛋白
  • 貨號：
    CSB-EP333015SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MEK1重組蛋白
  • 貨號：
    CSB-BP333015SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MEK1重組蛋白
  • 貨號：
    CSB-MP333015SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MEK1
  • Uniprot No.：
  • 別名：
    MEK1; MRE4; YOR351C; O6357; Meiosis-specific serine/threonine-protein kinase MEK1; EC 2.7.11.1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-497
  • 氨基酸序列
    MRPLYSCNLA TKDDIEMAGG VAPAHLEVNV GGYNTEQTIP IVKHQLVKVG RNDKECQLVL TNPSISSVHC VFWCVFFDED SIPMFYVKDC SLNGTYLNGL LLKRDKTYLL KHCDVIELSQ GSEENDIKKT RLVFMINDDL QSSLDPKLLD QMGFLREVDQ WEITNRIVGN GTFGHVLITH NSKERDEDVC YHPENYAVKI IKLKPNKFDK EARILLRLDH PNIIKVYHTF CDRNNHLYIF QDLIPGGDLF SYLAKGDCLT SMSETESLLI VFQILQALNY LHDQDIVHRD LKLDNILLCT PEPCTRIVLA DFGIAKDLNS NKERMHTVVG TPEYCAPEVG FRANRKAYQS FSRAATLEQR GYDSKCDLWS LGVITHIMLT GISPFYGDGS ERSIIQNAKI GKLNFKLKQW DIVSDNAKSF VKDLLQTDVV KRLNSKQGLK HIWIAKHLSQ LERLYYKKIL CNNEGPKLES INSDWKRKLP KSVIISQAIP KKKKVLE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Probable protein kinase required for meiotic recombination.
  • 基因功能參考文獻(xiàn)：
    1. ndt80 transcription of CDC5 results in degradation of Red1, a meiosis-specific protein required for Mek1 activation, thereby abolishing Mek1 activity completely. Elimination of Mek1 kinase activity allows Rad51-mediated repair of any remaining DSBs. In this way, cells do not enter Meiosis I until recombination is complete and all DSBs are repair PMID: 28249986
    2. When Mek1 persists on closely paired (synapsed) homologues, DNA repair is severely delayed, suggesting that Mek1 suppresses any proximal repair template. PMID: 26870961
    3. chromatin remodeling allows Dot1-mediated H3K79-me to exclude Pch2 from the chromosomes, thus driving localization of Hop1 along chromosome axes and enabling Mek1 full activation to trigger downstream responses, such as meiotic arrest PMID: 23382701
    4. propose that Mek1 plays dual roles during meiotic prophase I by phosphorylating targets directly involved in the recombination checkpoint, as well as targets involved in sister chromatid recombination PMID: 20888230
    5. Hop1 phosphorylation in response to double strand breakage triggers dimerization of Mek1. PMID: 16221890

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  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHEK2 subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YOR351C

    STRING: 4932.YOR351C



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