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  4. Recombinant Saccharomyces cerevisiae Kinesin-like protein KIP3 (KIP3), partial

Recombinant Saccharomyces cerevisiae Kinesin-like protein KIP3 (KIP3), partial

  • 中文名稱：
    釀酒酵母KIP3重組蛋白
  • 貨號：
    CSB-YP345253SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母KIP3重組蛋白
  • 貨號：
    CSB-EP345253SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母KIP3重組蛋白
  • 貨號：
    CSB-EP345253SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母KIP3重組蛋白
  • 貨號：
    CSB-BP345253SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母KIP3重組蛋白
  • 貨號：
    CSB-MP345253SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    KIP3
  • Uniprot No.：
  • 別名：
    KIP3; YGL216WKinesin-like protein KIP3
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 基因功能參考文獻：
    1. The single kinesin-8 motor Kip3 alternately mediates both catastrophe and rescue of the bud microtubule (MT). Kip3 accumulates in a length-dependent manner along the lattice of MTs within the bud, yet induces catastrophe spatially near the bud tip. PMID: 25088560
    2. Kip3 directly suppresses spindle microtubule polymerization, limiting midzone length. PMID: 24616221
    3. the motion of yeast (Kip3) and human (Kif18A) kinesin-8s PMID: 23746518
    4. antiparallel microtubule-sliding activity of the budding yeast kinesin-8, Kip3 PMID: 23851487
    5. Studies indicate that the motor protein Kip3 (in the kinesin-8 family) is an ideal model system to reconstitute high-density traffic on microtubules. PMID: 22431622
    6. A model in which TORC1 and Clb5/CDK act coordinately to promote both spindle assembly via a pathway involving Kip3 and S phase progression. PMID: 20697716
    7. Kip3p is a highly processive, plus-end-directed motor, both in vitro and in vivo. PMID: 16906145
    8. Kip3p plays an essential role in positioning the mitotic spindle in budding yeast and potentially other processes controlled by kinesin-8 family members. PMID: 16906148
    9. characterization of frictional drag force of KIP3(kinesin-8 motor proteins) interacting with microtubule tracks; argue these frictional forces arise from breaking bonds between motor domains & the microtubule & they limit speed & efficiency of kinesin PMID: 19679813
    10. Study found that after binding at a random position on a microtubule and walking to the plus end, an individual Kip3p molecule pauses there until an incoming Kip3p molecule bumps it off. PMID: 19766569

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin II subfamily
  • 數據庫鏈接：

    KEGG: sce:YGL216W

    STRING: 4932.YGL216W



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