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Recombinant Saccharomyces cerevisiae Kinesin-like protein KAR3 (KAR3), partial

  • 中文名稱:
    釀酒酵母KAR3重組蛋白
  • 貨號:
    CSB-YP322336SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母KAR3重組蛋白
  • 貨號:
    CSB-EP322336SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母KAR3重組蛋白
  • 貨號:
    CSB-EP322336SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母KAR3重組蛋白
  • 貨號:
    CSB-BP322336SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母KAR3重組蛋白
  • 貨號:
    CSB-MP322336SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    KAR3
  • Uniprot No.:
  • 別名:
    KAR3; YPR141C; P9659.16; Kinesin-like protein KAR3; Nuclear fusion protein
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Essential for yeast nuclear fusion during mating. KAR3 is a bifunctional protein having a kinesin-like motor domain joined to a distinct microtubule binding domain. It may mediate microtubule sliding during nuclear fusion and possibly mitosis. May interact with spindle microtubules to produce an inwardly directed force acting upon the poles. KAR3 function antagonizes CIP8 and KIP1 outward force action. KAR3 motor activity is directed toward the microtubule's minus end.
  • 基因功能參考文獻:
    1. findings provide an example for a non-conventional translocation mechanism and can explain how Kar3 substitutes for key functions of Dynein in the yeast nucleus PMID: 25626168
    2. Kar3, with the help of its light chain, Cik1, is anchored during mating to the SPB component Spc72 that also serves as a nucleator and anchor for microtubules via their minus ends. PMID: 23388829
    3. the kar3 meiotic arrest may be mediated by the spindle checkpoint PMID: 14726698
    4. In Kar3, the extended alpha-helical domain NH2-terminal to the catalytic core provides the structural transitions in response to the ATPase cycle that are critical for motility; dimerization is not specifically required PMID: 15385545
    5. Kinetochores slide along the microtubule lateral surface, which is mainly and probably exclusively driven by Kar3. PMID: 17620411
    6. Kar3p contributes to spindle stability by cross-linking spindle microtubules. PMID: 18180364
    7. Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating. PMID: 19386762

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  • 亞細胞定位:
    Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Nucleus. Cytoplasm, cytoskeleton. Note=Cytoplasmic microtubules.
  • 蛋白家族:
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, NCD subfamily
  • 數據庫鏈接:

    KEGG: sce:YPR141C

    STRING: 4932.YPR141C



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