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  4. Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8 (CIN8), partial

Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8 (CIN8), partial

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8(CIN8) ,partial
  • 貨號：
    CSB-YP338877SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8(CIN8) ,partial
  • 貨號：
    CSB-EP338877SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8(CIN8) ,partial
  • 貨號：
    CSB-EP338877SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8(CIN8) ,partial
  • 貨號：
    CSB-BP338877SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Kinesin-like protein CIN8(CIN8) ,partial
  • 貨號：
    CSB-MP338877SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CIN8
  • Uniprot No.：
  • 別名：
    CIN8; KSL2; YEL061CKinesin-like protein CIN8; Chromosome instability protein 8
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Required for assembly of the mitotic spindle. Interacts with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediated by KAR3.
  • 基因功能參考文獻(xiàn)：
    1. Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement PMID: 29117528
    2. Data suggest that approximately four Cin8 motor domains can bind each alpha/beta-tubulin dimer within microtubule lattice; Cin8 binds microtubules not only at the canonical site, but also one or more separate "non-canonical" sites. PMID: 28701465
    3. The tail domain is one of the factors that regulate Cin8 directionality. PMID: 25991727
    4. The deletion mutant cells exhibited reduced midzone-directed motility and efficiency to support spindle elongation, indicating the importance of directionality control for the anaphase function of Cin8. PMID: 22101328
    5. Dephosphorylation of Cin8 promotes its binding to the spindle microtubules before the onset of anaphase. PMID: 21378308
    6. Cin8 can switch between two states of directional motility. On individual microtubules, Cin8 motors move toward the minus end, they switch to plus-end movement when part of a team of mechanically coupled motors cross-linking antiparallel microtubules. PMID: 21350123
    7. Data suggest that spindle disassembly is achieved by mechanistically distinct but functionally overlapping subprocesses, which are largely governed by the anaphase-promoting complex, Aurora B kinase, and kinesin-8. PMID: 21079246
    8. Results suggest that binding of microtubules is not sufficient for the functionality of Cin8p and that microtubule cross-linking by the tetrameric complex is essential for Cin8p mitotic functions. PMID: 16829678
    9. Spindle elongation in anaphase in Saccharomyces cerevisiae cells mutated for the kinesin-5 motor protein Cin8 PMID: 18628309
    10. Data observed that deletion of Cin8, a microtubule-crosslinking kinesin-5 motor protein, reduced the recovery rate in anaphase spindles, indicating that Cin8 contributes to the destabilization of iMT plus-ends. PMID: 19270715

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  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton, spindle. Mitochondrion. Note=Spindle microtubules that lie between the poles.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, BimC subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YEL061C

    STRING: 4932.YEL061C



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