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  4. Recombinant Saccharomyces cerevisiae Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial (IDH2)

Recombinant Saccharomyces cerevisiae Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial (IDH2)

  • 中文名稱(chēng)：
    釀酒酵母IDH2重組蛋白
  • 貨號(hào)：
    CSB-YP340961SVG
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母IDH2重組蛋白
  • 貨號(hào)：
    CSB-EP340961SVG
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母IDH2重組蛋白
  • 貨號(hào)：
    CSB-EP340961SVG-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母IDH2重組蛋白
  • 貨號(hào)：
    CSB-BP340961SVG
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母IDH2重組蛋白
  • 貨號(hào)：
    CSB-MP340961SVG
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    IDH2; YOR136W; O3326; YOR3326W; Isocitrate dehydrogenase [NAD] subunit 2; mitochondrial; EC 1.1.1.41; Isocitric dehydrogenase; NAD(+)-specific ICDH
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    16-369
  • 氨基酸序列
    ATVKQ PSIGRYTGKP NPSTGKYTVS FIEGDGIGPE ISKSVKKIFS AANVPIEWES CDVSPIFVNG LTTIPDPAVQ SITKNLVALK GPLATPIGKG HRSLNLTLRK TFGLFANVRP AKSIEGFKTT YENVDLVLIR ENTEGEYSGI EHIVCPGVVQ SIKLITRDAS ERVIRYAFEY ARAIGRPRVI VVHKSTIQRL ADGLFVNVAK ELSKEYPDLT LETELIDNSV LKVVTNPSAY TDAVSVCPNL YGDILSDLNS GLSAGSLGLT PSANIGHKIS IFEAVHGSAP DIAGQDKANP TALLLSSVMM LNHMGLTNHA DQIQNAVLST IASGPENRTG DLAGTATTSS FTEAVIKRL
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Performs an essential role in the oxidative function of the citric acid cycle. Also binds RNA; specifically to the 5'-untranslated leaders of mitochondrial mRNAs.
  • 基因功能參考文獻(xiàn)：
    1. On the basis of a potential interaction between side chains of Cys-150 residues in IDH2 subunits in each tetramer of the enzyme, ligand binding assays of wild-type (IDH1/IDH2) and IDH1/IDH2(C150S) octameric enzymes were conducted. PMID: 21861471
    2. analysis of allosteric motion in the IDH1 and IDH2 subunits of NAD+-specific isocitrate dehydrogenase PMID: 18256028
    3. Structural studies of Saccharomyces cerevisiae mitochondrial NADP-dependent IDH2 in different enzymatic states reveal substantial conformational changes during the catalytic reactiion. PMID: 18552125
    4. Structural features of a ligand-free form of IDH2 suggest that formation of a disulfide bond during stationary phase in yeast transformants might stabilize an inactive form of the enzyme. PMID: 19645416
  • 亞細(xì)胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    Isocitrate and isopropylmalate dehydrogenases family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YOR136W

    STRING: 4932.YOR136W



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