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  4. Recombinant Saccharomyces cerevisiae ISWI chromatin-remodeling complex ATPase ISW1 (ISW1), partial

Recombinant Saccharomyces cerevisiae ISWI chromatin-remodeling complex ATPase ISW1 (ISW1), partial

  • 中文名稱(chēng)：
    釀酒酵母ISW1重組蛋白
  • 貨號(hào)：
    CSB-YP327786SVG
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母ISW1重組蛋白
  • 貨號(hào)：
    CSB-EP327786SVG
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母ISW1重組蛋白
  • 貨號(hào)：
    CSB-EP327786SVG-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母ISW1重組蛋白
  • 貨號(hào)：
    CSB-BP327786SVG
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    釀酒酵母ISW1重組蛋白
  • 貨號(hào)：
    CSB-MP327786SVG
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ISW1
  • Uniprot No.：
  • 別名：
    ISW1; YBR245C; YBR1633; ISWI chromatin-remodeling complex ATPase ISW1; EC 3.6.4.-
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.
  • 基因功能參考文獻(xiàn)：
    1. single mutations in genes ISW1, ISW2, and CHD1 weakly affect repair and mutagenic processes in yeast cells. PMID: 29372964
    2. Study identifies the yeast ISW1 chromatin remodeling complex as an unanticipated mRNP nuclear export surveillance factor that retains export-incompetent transcripts near their transcription site. This tethering activity of ISW1 requires chromatin binding and is independent of nucleosome sliding activity or changes in RNA polymerase II processivity. PMID: 27863241
    3. The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism. PMID: 25821983
    4. nucleosome mobilizing activity of Isw1a, Isw1b and Isw2 complexes could be significantly influenced by neighboring nucleosomes PMID: 24530316
    5. The nuclease digestion data suggest that Isw1a can also promote ATP-dependent distortion of nucleosome structure, although less efficiently than the Isw1b complex. PMID: 24050724
    6. nuclear localization signal is located at the end of the ISW1 C-terminus. PMID: 23121014
    7. We find that remodelers( Isw1, Isw2, and Chd1 ) occupancy at NDRs and gene bodies is associated with nucleosome turnover and transcriptional elongation rate PMID: 23468649
    8. Distinct ISWI-family members translocate nucleosomes with a similar stepping pattern maintained by the catalytic subunit of the enzyme. Nucleosome remodeling begins with a 7 bp step of DNA translocation followed by 3 bp subsequent steps toward the exit side of nucleosomes. PMID: 23374341
    9. Deletion of ISW1 and CHD1 causes widespread intragenic transcription. PMID: 22922743
    10. Histone H3 lysine 36 methylation promoted the chromatin interaction of the Isw1b chromatin-remodeling complex in Saccharomyces cerevisiae. PMID: 22751925
    11. fndings show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome PMID: 21940898
    12. ISWI and Chd1 remodelling factors are not only involved in transcription-related chromatin remodelling, but also are required to maintain a specific chromatin configuration across the yeast genome. PMID: 16468993
    13. ISWI and Chd1 chromatin remodeling complexes have roles in nucleosome repositioning PMID: 16606615
    14. Data support a model in which Isw1 acts independently of the previously characterized Isw1a and Isw1b complexes to maintain a heterochromatin-like structure at the rDNA that is required for gene silencing. PMID: 17561109
    15. Data show that, although defective in nucleosome remodeling, Isw1 lacking the SANT domain is able to repress transcription initiation at the MET16 promoter. PMID: 19273607

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    SNF2/RAD54 helicase family, ISWI subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YBR245C

    STRING: 4932.YBR245C



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