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  4. Recombinant Saccharomyces cerevisiae Histone demethylase JHD2 (JHD2), partial

Recombinant Saccharomyces cerevisiae Histone demethylase JHD2 (JHD2), partial

  • 中文名稱：
    釀酒酵母JHD2重組蛋白
  • 貨號：
    CSB-YP344439SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母JHD2重組蛋白
  • 貨號：
    CSB-EP344439SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母JHD2重組蛋白
  • 貨號：
    CSB-EP344439SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母JHD2重組蛋白
  • 貨號：
    CSB-BP344439SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母JHD2重組蛋白
  • 貨號：
    CSB-MP344439SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    JHD2
  • Uniprot No.：
  • 別名：
    JHD2; YJR119C; J2035Histone demethylase JHD2; EC 1.14.11.-; Jumonji/ARID domain-containing protein 2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated H3 'Lys-4'.
  • 基因功能參考文獻：
    1. H2B Lys-123 ubiquitination blocks Jhd2 from accessing its binding site on chromatin, and thereby, we have uncovered a second mechanism by which H2B ubiquitination contributes to the trans-histone regulation of H3K4 methylation. PMID: 26451043
    2. Jhd2, an evolutionarily conserved JARID1 family H3 Lys4 demethylase, effects all states of methylated histone H3K4 within the NTS regions of rDNA, and its activity is required for the regulation of rDNA silencing in a Sir2-independent manner. PMID: 25248920
    3. the JmjN domain of Jhd2 is important for its protein stability, and the plant homeodomain (PHD) finger mediates its chromatin association independent of H3K4 methylation PMID: 20538609
    4. A novel budding yeast JmjC domain-containing H3-K4 demethylase, Jhd2p, that antagonizes the trimethyl modification state and contributes to regulation of telomeric silencing. PMID: 17310254
    5. Ectopic overexpression of mouse Jarid1B, a Yjr119Cp homolog, greatly diminished H3-(lysine) K4me3 and H3-K4me2 in HeLa cells, suggesting these proteins function as K4 demethylases in vivo. PMID: 17310255
    6. the protein level of the yeast histone H3 Lys 4 (H3 K4) demethylase Jhd2/Kdm5 is modulated through polyubiquitination by the E3 ubiquitin ligase Not4 and turnover by the proteasome PMID: 19346402

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    JARID1 histone demethylase family
  • 數據庫鏈接：

    KEGG: sce:YJR119C

    STRING: 4932.YJR119C



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