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  4. Recombinant Saccharomyces cerevisiae Low glucose sensor SNF3 (SNF3), partial

Recombinant Saccharomyces cerevisiae Low glucose sensor SNF3 (SNF3), partial

  • 中文名稱：
    釀酒酵母SNF3重組蛋白
  • 貨號：
    CSB-YP319940SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SNF3重組蛋白
  • 貨號：
    CSB-EP319940SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SNF3重組蛋白
  • 貨號：
    CSB-EP319940SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SNF3重組蛋白
  • 貨號：
    CSB-BP319940SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SNF3重組蛋白
  • 貨號：
    CSB-MP319940SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SNF3
  • Uniprot No.：
  • 別名：
    SNF3; YDL194W; D1234; Low glucose sensor SNF3; High-affinity glucose receptor SNF3; High-affinity transporter-like sensor SNF3; Sucrose nonfermenting protein 3
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    High-affinity low glucose sensor that is part of the sensor/receptor-repressor (SSR) glucose-signaling pathway, which detects extracellular glucose and induces expression of glucose transporters that bring glucose into the cell. The transporter-like sensor generates an intracellular signal in the presence of low levels of glucose to promote low glucose-induced expression of HXT2. Binding of glucose to the SNF3 transmembrane domain activates a downstream signaling cascade, leading to phosphorylation of the RGT1 corepressors MTH1 and STD1, targeting them for SCF(Grr1)-dependent ubiquitination and degradation. Depletion of the corepressors robs RGT1 of its ability to repress expression of HXT genes, leading to accumulation of glucose transporters in the plasma membrane. SNF3 is involved as well in the transport of mannose and fructose. Even though SNF3 is similar to glucose transporters, it appears to be unable to transport glucose.
  • 基因功能參考文獻：
    1. Rgt2 and Snf3 glucose sensors are primary targets of methylglyoxal, which causes degradation of the Rgt2 and Snf3. PMID: 26764094
    2. Mth1 and Std1, which are downstream effectors of the Snf3/Rgt2 pathway, were required for viability through mitochondrial function but not fermentative metabolism PMID: 25541485
    3. Snf3 apparent affinity for glucose was lowest when the intracellular glucose concentration was high. The results conform to an alternating-access model for transporter-like sensors PMID: 20564191
    4. This study reveals that Snf3, in addition to glucose, also senses fructose and mannose, as well as the glucose analogues 2-deoxyglucose, 3-O-methylglucoside and 6-deoxyglucose. PMID: 20014043
    5. Suppression of the snf3 mutant phenotype by deletion of IRA2 was confirmed. PMID: 17311585

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family
  • 數據庫鏈接：

    KEGG: sce:YDL194W

    STRING: 4932.YDL194W



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