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  4. Recombinant Saccharomyces cerevisiae Glycerol uptake/efflux facilitator protein (FPS1), partial

Recombinant Saccharomyces cerevisiae Glycerol uptake/efflux facilitator protein (FPS1), partial

  • 中文名稱：
    釀酒酵母FPS1重組蛋白
  • 貨號(hào)：
    CSB-YP326102SVG1
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母FPS1重組蛋白
  • 貨號(hào)：
    CSB-EP326102SVG1
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母FPS1重組蛋白
  • 貨號(hào)：
    CSB-EP326102SVG1-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母FPS1重組蛋白
  • 貨號(hào)：
    CSB-BP326102SVG1
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母FPS1重組蛋白
  • 貨號(hào)：
    CSB-MP326102SVG1
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    FPS1; YLL043W; Glycerol uptake/efflux facilitator protein
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Channel protein for glycerol. Has a role in both glycerol influx and efflux. Plays a role in osmoregulation: under osmotic stress the channel is apparently closed to allow accumulation of glycerol in the cell under hyperosmotic conditions.
  • 基因功能參考文獻(xiàn)：
    1. Report shows that Slt2 is phosphorylated following arsenite treatment. Slt2 and its kinase activity are necessary for full acquisition of arsenite tolerance and this effect appears to be mediated through Fps1. Hence, it appears that two MAPKs, Hog1 and Slt2, collaborate in controlling arsenite influx and efflux through Fps1 suggesting a mechanism where this MAPK collaboration may function on Fps1. PMID: 27607883
    2. Moreover, hyperosmotic conditions block TORC2-dependent Ypk1-mediated Fps1 phosphorylation, causing channel closure, glycerol accumulation, and enhanced survival under hyperosmotic stress. PMID: 26274562
    3. results show the function of Stl1p and Fps1p transporters contributes to cell's ability to survive during transfer into state of anhydrobiosis; deletion of FPS1 decreases cell's tolerance of hyperosmotic stress; deletion of STL1 results in slight increase in cell size and increase in intracellular pH PMID: 25673653
    4. Fps1 is key for downstream signaling in the Sho1-mediated branch of the high-osmolarity glycerol mitogen-activated protein kinase pathway pathway. PMID: 25644660
    5. Activation of Hog1 in response to hyperosmotic shock induces the rapid eviction of Rgc2 from Fps1 and consequent channel closure PMID: 24298058
    6. Data suggest a mechanism for regulation of glycerol flux in yeast, where the termini alone are not sufficient to restrict Fps1 transport. PMID: 22593571
    7. Fps1 functions as a homotetramer to regulate glycerol transport in yeast. PMID: 22030956
    8. Data show that Fps1p in concert with the arsenite transporter Acr3p mediates arsenite efflux. PMID: 20026328
    9. Hog1 was also required for basal phosphorylation of ASK10, suggesting a mechanism by which Hog1 may regulate Fps1 indirectly PMID: 19956799
    10. We showed that increased arsenite influx is dependent on the aquaglyceroporin Fps1p. Fps1p is phosphorylated on threonine 231 in vivo and this phosphorylation critically affects Fps1p activity. Moreover, Hog1p is shown to affect Fps1p phosphorylation. PMID: 16885417
    11. These results suggest that, in addition to the efflux boron transporter BOR1, DUR3 and FPS1 play important roles in regulating the cellular boron concentration. PMID: 16923078
    12. Improved production of ethanol by deleting FPS1. PMID: 17043906
    13. HOG1 phosphorylation targets the FPS1 aqualglyceroporin for endocytosis, thereby rendering cells resistant to acetic acid. PMID: 17620418
    14. Lack of Fps1p therefore exerts opposing effects on the activation of Hog1p and Slt2p in yeast exposed to acetic acid stress. PMID: 19608606
    15. FPS1 expression contributes to decreased [(3)H]ethanol accumulation in yeast cells, suggesting that Fps1p may also play a role in maintaining the intracellular ethanol level during active fermentation. PMID: 19633105

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  • 亞細(xì)胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YLL043W

    STRING: 4932.YLL043W



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