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  4. Recombinant Saccharomyces cerevisiae Glutaredoxin-2, mitochondrial (GRX2)

Recombinant Saccharomyces cerevisiae Glutaredoxin-2, mitochondrial (GRX2)

  • 中文名稱：
    釀酒酵母GRX2重組蛋白
  • 貨號：
    CSB-YP322384SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母GRX2重組蛋白
  • 貨號：
    CSB-EP322384SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母GRX2重組蛋白
  • 貨號：
    CSB-EP322384SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母GRX2重組蛋白
  • 貨號：
    CSB-BP322384SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母GRX2重組蛋白
  • 貨號：
    CSB-MP322384SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GRX2
  • Uniprot No.：
  • 別名：
    GRX2; TTR; TTR1; YDR513W; D9719.17Glutaredoxin-2; EC 1.11.1.9; EC 2.5.1.18; Glutathione-dependent oxidoreductase 2; Thioltransferase
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    36-143
  • 氨基酸序列
    VSQET VAHVKDLIGQ KEVFVAAKTY CPYCKATLST LFQELNVPKS KALVLELDEM SNGSEIQDAL EEISGQKTVP NVYINGKHIG GNSDLETLKK NGKLAEILKP VFQ
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the glutathione system which performs several activities such as glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase (GST) activity. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing cytosolic protein- and non-protein-disulfides in a coupled system with glutathione reductase. Required for resistance to reactive oxygen species (ROS) by directly reducing hydroperoxides and for the detoxification of ROS-mediated damage. GRX2 is more active as an oxidoreductase than GRX1. Responsible for the S-glutathionylation of DHBP synthase.
  • 基因功能參考文獻(xiàn)：
    1. Data show that the gatekeeper function of glutaredoxin Grx2 is dependent on its concentration in the mitochondrial intermembrane space (IMS). PMID: 25392302
    2. specific functions of two interacting redoxins: dually localized glutaredoxin 2 (Grx2p) and mitochondrial peroxiredoxin 1 (Prx1p) PMID: 21385868
    3. Results suggest that although Trx is the reductant involved in the reduction of peroxides by 2-Cys-peroxiredoxin (Prx), glutaredoxin 2 might be the natural resolving partner of 1-Cys Prx through a monothiolic mechanism. PMID: 20059400
    4. Mutations on key basic residues drastically affect the cellular fate of the protein, showing that evolutionary diversification of Grx2 structural and functional properties are strictly dependent on the sequence of the targeting signal peptide. PMID: 20036764
    5. a plethora of Grx2 subcellular localizations could spread its antioxidant functions all over the cell, but one single Ala to Gly mutation converts Grx2 into a typical protein of the mitochondrial matrix PMID: 16606613
    6. Grx2 is involved with the apoptotic death induced by cadmium, a form of cellular suicide that might lead of removal of mutated cells. PMID: 17996374
    7. glutaredoxin 2 and cytosolic thioredoxins have roles in cysteinyl-based redox modification of the 20S proteasome PMID: 18435761

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  • 亞細(xì)胞定位：
    [Isoform Cytoplasmic]: Cytoplasm.; [Isoform Mitochondrial]: Mitochondrion.
  • 蛋白家族：
    Glutaredoxin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YDR513W

    STRING: 4932.YDR513W



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