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  4. Recombinant Saccharomyces cerevisiae Glutamate--tRNA ligase, cytoplasmic (GUS1), partial

Recombinant Saccharomyces cerevisiae Glutamate--tRNA ligase, cytoplasmic (GUS1), partial

  • 中文名稱：
    釀酒酵母GUS1重組蛋白
  • 貨號：
    CSB-YP342816SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母GUS1重組蛋白
  • 貨號：
    CSB-EP342816SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母GUS1重組蛋白
  • 貨號：
    CSB-EP342816SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母GUS1重組蛋白
  • 貨號：
    CSB-BP342816SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母GUS1重組蛋白
  • 貨號：
    CSB-MP342816SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GUS1
  • Uniprot No.：
  • 別名：
    GUS1; YGL245W; G0583; HRB724; Glutamate--tRNA ligase; cytoplasmic; EC 6.1.1.17; Glutamyl-tRNA synthetase,; c)ERS; GluRS; P85
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). In mitochondria, constitutes the nondiscriminating glutamyl-tRNA synthase that generates the mitochondrial mischarged glutamyl-tRNA(Gln) substrate for the tRNA-dependent amidotransferase (AdT), which generates mitochondrial glutaminyl-tRNA(Gln) by transamidation of glutamyl-tRNA(Gln).
  • 基因功能參考文獻(xiàn)：
    1. the eukaryote-specific protein domains of GluRS and GlnRS substantially influence amino acid binding, tRNA binding and aminoacylation efficiency, but they play no role in either specific nucleotide readout or discrimination against noncognate tRNA PMID: 25149203
    2. These results are consistent with a model according to which Arc1p is a bifunctional protein involved in the subcellular localization of ScMetRS and ScGluRS via its N-terminal domain and of tRNA via its C-terminal domain. PMID: 17131041
    3. The structure of the interacting domain of yeast glutamyl-tRNA synthetase (GluRS) and its accessory protein Arc1p was determined and refined to 1.9 A resolution. PMID: 17139087
    4. assembly of the Arc1p-synthetase complex is mediated exclusively by the N-terminal domains of the synthetases and that the two enzymes bind to largely independent sites on Arc1p. PMID: 17976650
    5. cytosolic glutamyl-tRNA synthetase ((c)ERS) is imported into mitochondria, where it constitutes the mitochondrial nondiscriminating ERS that generates the mitochondrial mischarged glutamyl-tRNA(Gln) substrate for the AdT PMID: 19417106

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  • 亞細(xì)胞定位：
    Cytoplasm. Mitochondrion. Note=Largely excluded from the nucleus.
  • 蛋白家族：
    Class-I aminoacyl-tRNA synthetase family, Glutamate--tRNA ligase type 2 subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YGL245W

    STRING: 4932.YGL245W



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