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Recombinant Saccharomyces cerevisiae GTP-binding protein RHO1 (RHO1)

  • 中文名稱:
    釀酒酵母RHO1重組蛋白
  • 貨號:
    CSB-YP361944SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母RHO1重組蛋白
  • 貨號:
    CSB-EP361944SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母RHO1重組蛋白
  • 貨號:
    CSB-EP361944SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母RHO1重組蛋白
  • 貨號:
    CSB-BP361944SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母RHO1重組蛋白
  • 貨號:
    CSB-MP361944SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    RHO1
  • Uniprot No.:
  • 別名:
    RHO1; YPR165W; P9325.3; GTP-binding protein RHO1; Rho-type GTPase 1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Full Length of Mature Protein
  • 表達區域:
    2-206
  • 氨基酸序列
    SQQVGNSIR RKLVIVGDGA CGKTCLLIVF SKGQFPEVYV PTVFENYVAD VEVDGRRVEL ALWDTAGQED YDRLRPLSYP DSNVVLICFS IDLPDSLENV QEKWIAEVLH FCQGVPIILV GCKVDLRNDP QTIEQLRQEG QQPVTSQEGQ SVADQIGATG YYECSAKTGY GVREVFEAAT RASLMGKSKT NGKAKKNTTE KKKKKC
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Acts as a central regulator in the cell wall integrity signaling pathway, which is regulated by the cell cycle and in response to various types of cell wall stress. Integrates signals from different cell surface sensors, and activates a set of effectors, regulating processes including beta-glucan synthesis at the site of wall remodeling, gene expression related to cell wall biogenesis, organization of the actin cytoskeleton, and protein- and secretory vesicle-targeting to the growth site. Activates the protein kinase C (PKC1) MAP kinase cascade, the beta-1,3-glucan synthase (FKS1), the formin BNI1, the exocyst component SEC3 and the transcription factor SKN7.
  • 基因功能參考文獻:
    1. The signaling network of Rho1 extends into many TORC1-dependent processes and sheds light on how yeast cells coordinate polarized spatial expansion with mass increase. PMID: 22918240
    2. synthesis of the cell wall through partitioning glucose to the periphery and the formation of the signaling complex with Rom2 and Ssd1 to promote Rho1-dependent polarized cell growth. PMID: 22296835
    3. TOR complex 1 acts both upstream and downstream of Rho1 GTPase, unveiling a mechanism that integrates stress and nutrient signals to coordinate Rho1-mediated spatial expansion and TORC1-dependent mass increase. PMID: 22445487
    4. The result reveal the existence of a second pathway for endocytosis in yeast, which suggests similarities with the RhoA-dependent endocytic pathways of mammalian cells. PMID: 22065638
    5. Rho1 protects yeast cells from oxidative stress by regulating multiple downstream targets including Ycf1. PMID: 21625004
    6. Residues Phe77, Ile115 and Leu131 of Sec3 bind to an extended hydrophobic surface formed around switch regions I and II of Rho1. PMID: 20062059
    7. Rho1p regulates the assembly state of actin on the peroxisome membrane and is specifically recruited to peroxisomes upon their induction in a process dependent on its interaction with the peroxisome membrane protein Pex25p PMID: 15596542
    8. Morphological analyses have revealed that the small GTPase Rho1p participates in cell-size control at both the G1/S and the G2/M boundaries. PMID: 17302939
    9. budding yeast utilizes two mechanisms to concentrate Rho1 at the division site PMID: 19339687
    10. A MAP kinase dependent feedback mechanism controls Rho1 GTPase and actin distribution PMID: 19564916

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  • 亞細胞定位:
    Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Peroxisome membrane; Lipid-anchor. Note=Plasma membrane-associated at sites of polarized growth such as incipient bud sites, bud tips, the bud neck during cytokinesis, and the neck and tip of mating projections. Also found on internal membranes of endosomes and peroxisomes.
  • 蛋白家族:
    Small GTPase superfamily, Rho family
  • 數據庫鏈接:

    KEGG: sce:YPR165W

    STRING: 4932.YPR165W



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