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  4. Recombinant Saccharomyces cerevisiae G2/mitotic-specific cyclin-2 (CLB2)

Recombinant Saccharomyces cerevisiae G2/mitotic-specific cyclin-2 (CLB2)

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae G2/mitotic-specific cyclin-2(CLB2)
  • 貨號：
    CSB-YP326178SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae G2/mitotic-specific cyclin-2(CLB2)
  • 貨號：
    CSB-EP326178SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae G2/mitotic-specific cyclin-2(CLB2)
  • 貨號：
    CSB-EP326178SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae G2/mitotic-specific cyclin-2(CLB2)
  • 貨號：
    CSB-BP326178SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae G2/mitotic-specific cyclin-2(CLB2)
  • 貨號：
    CSB-MP326178SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CLB2
  • Uniprot No.：
  • 別名：
    CLB2; YPR119W; P9642.6G2/mitotic-specific cyclin-2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-491
  • 氨基酸序列
    SNPIENTEN SQNTSSSRFL RNVQRLALNN VTNTTFQKSN ANNPALTNFK STLNSVKKEG SRIPQFTRES VSRSTAAQEE KRTLKENGIQ LPKNNLLDDK ENQDPSSQQF GALTSIKEGR AELPANISLQ ESSSAKEIIQ HDPLKGVGSS TEVVHNSVEN EKLHPARSQL QVRNTESETD SGKKRPISTI VEQELPKKFK VCDENGKEEY EWEDLDAEDV NDPFMVSEYV NDIFEYLHQL EVITLPKKED LYQHRNIHQN RDILVNWLVK IHNKFGLLPE TLYLAINIMD RFLGKELVQL DKLQLVGTSC LFIASKYEEV YSPSIKHFAS ETDGACTEDE IKEGEKFILK TLKFNLNYPN PMNFLRRISK ADDYDIQSRT LAKFLLEISL VDFRFIGILP SLCAAAAMFM SRKMLGKGKW DGNLIHYSGG YTKEELAPVC HMIMDYLVSP IVHDEFHRKY QSRRFMKASI ISVQWALKVR KNGYDIMTLH E
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential for the control of the cell cycle at the G2/M (mitosis) transition. Interacts with the CDC2 protein kinase to form MPF. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis.
  • 基因功能參考文獻：
    1. Data indicate the role of the principal mitotic cyclin gene CLB2 at the end of S phase. PMID: 29279302
    2. Size control seems to be exerted twice, where the G2/M control affects bud size through bud-localized translation of CLB2 mRNA, explaining the dependence of the S-G2-M duration on nutrients. PMID: 25910075
    3. The essential role of nuclear Clb2 is in both survival and adaptation to the DNA damage checkpoint, illustrating a spatially distinct dual function of this mitotic cyclin in the response to DNA damage. PMID: 24300211
    4. Study discovered a pathway that links starvation to control of CLB2 mRNA stability and M phase delay. Dbf2 phosphorylates and activates the arginine methyltransferase Hmt1. In turn, Hmt1 methylates hnRNPs, promoting their nuclear localization, their cotranscriptional association with CLB2 mRNA, and the stability of CLB2 mRNA. PMID: 23706744
    5. These observations differentiate the functions of CLB1, CLB2, and CLB3 at mitotic exit.Actin ring formation is disrupted by deletion of CLB2 in cdc14-arrested cells PMID: 21784165
    6. The inhibition of mitotic exit in budding yeast using graded levels of stable mitotic cyclin (Clb2), is investigated. PMID: 19920813
    7. transcription factors for CLB2 are involved in the cell wall integrity checkpoint system and suggest that there are multiple regulation pathways of the checkpoint. PMID: 20057164
    8. CDK interaction with the amino-terminal domain of Cdc6 is mediated by the cyclin subunit Clb2 PMID: 15496876
    9. development of a model to generate biochemical predictions for Clb2 levels, asynchronous as well as through the cell cycle, as the Clb2 overexpression threshold is approached PMID: 15716353
    10. cytoplasmic Clb2is required for timely inactivation of Swe1 at the G2/M transition, and bud morphogenesis. PMID: 17033818
    11. The subcellular localization of the mitotic cyclin Clb2 is one of the key determinants of its biological function. PMID: 17264146
    12. functional antagonism between Rot1 and Clb2 is supported by genetic and biochemical data, which indicate that Rot1 is implicated in Clb2 protein degradation PMID: 17606994
    13. The inactivation of the Sin3 histone deacetylase complex bypasses the essential role of Ndd1 in cell cycle progression. Sin3 and its catalytic subunit Rpd3 associate with the CLB2 promoter during the G1 phase of the cell cycle. PMID: 17908798
    14. Cells expressing partially stable Clb2 were particularly sensitive to different stresses, possibly due to reduced Sic1 levels. PMID: 19713762

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  • 蛋白家族：
    Cyclin family, Cyclin AB subfamily
  • 數據庫鏈接：

    KEGG: sce:YPR119W

    STRING: 4932.YPR119W



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