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  4. Recombinant Saccharomyces cerevisiae F-box protein MET30 (MET30), partial

Recombinant Saccharomyces cerevisiae F-box protein MET30 (MET30), partial

  • 中文名稱：
    釀酒酵母MET30重組蛋白
  • 貨號：
    CSB-YP330966SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MET30重組蛋白
  • 貨號：
    CSB-EP330966SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MET30重組蛋白
  • 貨號：
    CSB-EP330966SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MET30重組蛋白
  • 貨號：
    CSB-BP330966SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MET30重組蛋白
  • 貨號：
    CSB-MP330966SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MET30
  • Uniprot No.：
  • 別名：
    MET30; YIL046W; F-box protein MET30; E3 ubiquitin ligase complex SCF(Met30) subunit MET30; Methionine-requiring protein 30
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Substrate-recognition component of the SCF(Met30) complex, an E3 ubiquitin ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the SCF(Met30) complex. Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Required for efficient expression of G1 cyclins. The SCF(Met30) complex catalyzes ubiquitination and degradation of the Cdk-inhibitory kinase SWE1. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. The SCF(Met30) complex mediates ubiquitination and subsequent degradation of MET4 and the cellular response to cadmium. The SCF(Met30) complex acts as an inhibitor of autophagy by promoting ubiquitination and degradation of ATG9 in normal conditions.
  • 基因功能參考文獻(xiàn)：
    1. In Saccharomyces cerevisiae, transcription of the MET regulon is controlled by ubiquitination of the transcription factor Met4, which is carried out by the SCF(Met30) E3 ubiquitin ligase. PMID: 24648496
    2. The stability of these DNA-binding cofactors is regulated by SCF(Met30). PMID: 21172660
    3. The association of Met30 with Skp1 but not with its substrate Met4 was inhibited in cells treated with cadmium PMID: 15689486
    4. data suggest that Met30 regulates several aspects of the cell cycle, including G(1)-specific transcription, initiation of DNA replication, and progression through M phase PMID: 15870262
    5. the recruitment of Met4p to the SCF(Met30p) complex itself results in inactivation of Met4p, independently of its ubiquitination PMID: 15883825

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    WD repeat MET30/SCONB/SCON-2 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YIL046W

    STRING: 4932.YIL046W



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