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  4. Recombinant Saccharomyces cerevisiae E3 ubiquitin-protein ligase UBR1 (UBR1), partial

Recombinant Saccharomyces cerevisiae E3 ubiquitin-protein ligase UBR1 (UBR1), partial

  • 中文名稱：
    釀酒酵母UBR1重組蛋白
  • 貨號：
    CSB-YP025516SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母UBR1重組蛋白
  • 貨號：
    CSB-EP025516SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母UBR1重組蛋白
  • 貨號：
    CSB-EP025516SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母UBR1重組蛋白
  • 貨號：
    CSB-BP025516SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母UBR1重組蛋白
  • 貨號：
    CSB-MP025516SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    UBR1
  • Uniprot No.：
  • 別名：
    UBR1; PTR1; YGR184C; G7168; E3 ubiquitin-protein ligase UBR1; EC 2.3.2.27; N-end-recognizing protein; N-recognin-1; RING-type E3 ubiquitin transferase UBR1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
  • 基因功能參考文獻(xiàn)：
    1. Ubr1 Is Present at the endoplasmic reticulum membrane of wild-type cells and becomes a ligase for ER-associated protein degradation of Ste6* under heat and ethanol stress. PMID: 23988329
    2. Study shows that the RING-type Ubr1 E3 and the HECT-type Ufd4 E3 interact, both physically and functionally. PMID: 21076411
    3. The UBR box, alone & in complex with N-degron peptides, has a previously unknown protein fold stabilized by a novel binuclear zinc center. N-terminal Arg, Lys or His N-degron side chains are coordinated in a multispecific binding pocket. PMID: 20835240
    4. Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway PMID: 18566452
    5. Yck1/Yck2-mediated phosphorylation of Ubr1 on Ser(300) plays a major role in the control of peptide import by the N-end rule pathway, the subsequent (primed) phosphorylations, including the one on Tyr(277), have at most minor effects on Ubr1 PMID: 19033468
    6. Ubr1 is responsible for targeting misfolded cytosoplasmic protein to proteasomal degradation. PMID: 19041308

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  • 蛋白家族：
    UBR1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YGR184C

    STRING: 4932.YGR184C



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