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Recombinant Saccharomyces cerevisiae E3 SUMO-protein ligase MMS21 (MMS21)

  • 中文名稱:
    釀酒酵母MMS21重組蛋白
  • 貨號:
    CSB-YP336471SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母MMS21重組蛋白
  • 貨號:
    CSB-EP336471SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母MMS21重組蛋白
  • 貨號:
    CSB-EP336471SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母MMS21重組蛋白
  • 貨號:
    CSB-BP336471SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母MMS21重組蛋白
  • 貨號:
    CSB-MP336471SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    MMS21
  • Uniprot No.:
  • 別名:
    MMS21; NSE2; YEL019C; E3 SUMO-protein ligase MMS21; EC 2.3.2.-; E3 SUMO-protein transferase MMS21; Methyl methanesulfonate-sensitivity protein 21; Non-structural maintenance of chromosome element 2; Non-SMC element 2
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-267
  • 氨基酸序列
    MALNDNPIPK SVPLHPKSGK YFHNLHARDL SNIYQQCYKQ IDETINQLVD STSPSTIGIE EQVADITSTY KLLSTYESES NSFDEHIKDL KKNFKQSSDA CPQIDLSTWD KYRTGELTAP KLSELYLNMP TPEPATMVNN TDTLKILKVL PYIWNDPTCV IPDLQNPADE DDLQIEGGKI ELTCPITCKP YEAPLISRKC NHVFDRDGIQ NYLQGYTTRD CPQAACSQVV SMRDFVRDPI MELRCKIAKM KESQEQDKRS SQAIDVL
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Acts as an E3 ligase mediating SUMO/Smt3 attachment to SMC5 and YKU70. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.
  • 基因功能參考文獻:
    1. Mms21 prevents the accumulation of spontaneous DNA lesions that cause diverse gross chromosomal rearrangements. PMID: 29505562
    2. Data indicate that Snf1 Is the target of SUMO (E3) ligase Mms21. PMID: 24108357
    3. Deletion of E3 ligase domain of Mms21 led to decreased binding of Sir2p, Sir3p and Sir4 at subtelomeric chromatins and increased H3K4 tri-methylation at telomere-distal euchromatin regions. PMID: 23911609
    4. Small ubiquitin-related modifier ligase activity of Mms21 is required for maintenance of chromosome integrity during the unperturbed mitotic cell division cycle in Saccharomyces cerevisiae PMID: 21324902
    5. Mms21 requires the Siz/PIAS (protein inhibitor of activated signal transducer and activator of transcription) RING domain to function as a SUMO ligase. PMID: 15738391
    6. Molecular cloning proved pso10-1 to be a mutant allele of gene MMS21 that encodes a SUMO ligase involved in the sumoylation of several DNA repair proteins. PMID: 18437386

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  • 亞細胞定位:
    Nucleus. Cytoplasm.
  • 蛋白家族:
    NSE2 family
  • 數據庫鏈接:

    KEGG: sce:YEL019C

    STRING: 4932.YEL019C



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