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Recombinant Saccharomyces cerevisiae Diphthamide biosynthesis protein 3 (KTI11)

  • 中文名稱:
    釀酒酵母KTI11重組蛋白
  • 貨號:
    CSB-YP661655SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母KTI11重組蛋白
  • 貨號:
    CSB-EP661655SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母KTI11重組蛋白
  • 貨號:
    CSB-EP661655SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母KTI11重組蛋白
  • 貨號:
    CSB-BP661655SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母KTI11重組蛋白
  • 貨號:
    CSB-MP661655SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    KTI11
  • Uniprot No.:
  • 別名:
    KTI11; DPH3; YBL071W-ADiphthamide biosynthesis protein 3; Kluyveromyces lactis toxin-insensitive protein 11
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-82
  • 氨基酸序列
    MSTYDEIEIE DMTFEPENQM FTYPCPCGDR FQIYLDDMFE GEKVAVCPSC SLMIDVVFDK EDLAEYYEEA GIHPPEPIAA AA
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2. Together with ATS1/KTI13; associates with the elongator and is required for tRNA Wobble base modifications mediated by the elongator complex. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine).
  • 基因功能參考文獻:
    1. The Diphthamide and the tRNA wobble uridine modifications both require diphthamide biosynthesis 3 (Dph3) protein as an electron donor for the iron-sulfur clusters in their biosynthetic enzymes. PMID: 27694803
    2. Solution structure of the Kti11 protein using NMR. Provides the first structure of the CSL-class zinc-binding protein family. PMID: 15952786
    3. Chemical and stereo structural information around the Zn ion in Kti11p was obtained high-spatial resolution technique MXAN. PMID: 18606152
    4. Kti11/Dph3 is involved in tRNA modification, tRNA suppression and prevents ADP-ribosylation of eEF2 by diphthamide. PMID: 18627462
  • 亞細胞定位:
    Cytoplasm. Nucleus.
  • 蛋白家族:
    DPH3 family
  • 數據庫鏈接:


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