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  4. Recombinant Saccharomyces cerevisiae Defective in cullin neddylation protein 1 (DCN1)

Recombinant Saccharomyces cerevisiae Defective in cullin neddylation protein 1 (DCN1)

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Defective in cullin neddylation protein 1(DCN1)
  • 貨號(hào)：
    CSB-YP619594SVG
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Defective in cullin neddylation protein 1(DCN1)
  • 貨號(hào)：
    CSB-EP619594SVG
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Defective in cullin neddylation protein 1(DCN1)
  • 貨號(hào)：
    CSB-EP619594SVG-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Defective in cullin neddylation protein 1(DCN1)
  • 貨號(hào)：
    CSB-BP619594SVG
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Defective in cullin neddylation protein 1(DCN1)
  • 貨號(hào)：
    CSB-MP619594SVG
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DCN1
  • Uniprot No.：
  • 別名：
    DCN1; YLR128W; L3111Defective in cullin neddylation protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-269
  • 氨基酸序列
    MSNNKIKRKD ASPEQEAIES FTSLTKCDPK VSRKYLQRNH WNINYALNDY YDKEIGTFTD EVSTVAHPPV YPKELTQVFE HYINNNLFDI DSLVKFIEEL GYNLEDLATL CLAHLLGYKK LEEPLKREDF LSTWFMQGCS TISDMQECIK TLDVKLHEDL QYFTQIYNYA FNLILDPNRK DIDTDEGIQY WKLFFQPEYP VRMEPDLLEA WFRFLRDEGK TTISKDTWRM LLLFFKRYPT IQKIISDYDE TAAWPFIIDE FYECLQDQQ
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes. Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity. Does not act by preventing deneddylation, but rather facilitates neddylation, possibly by acting with HRT1/RBX1 to recruit the Nedd8-charged E2 UBC12 to the cullin component of SCF-type complexes.
  • 基因功能參考文獻(xiàn)：
    1. Both in vivo and in vitro experiments indicate that Dcn1p does not inhibit deneddylation of Cdc53p by the COP9 signalosome, but greatly increases the kinetics of the neddylation reaction PMID: 15988528
    2. DCN-1 is a scaffold protein in a multisubunit neddylation E3 ligase complex PMID: 17597076
    3. The 1.9 A X-ray crystal structure of Dcn1 has an N-terminal ubiquitin-binding domain & a unique C-terminal domain. It does not require Cys for catalysis. It interacts with the Nedd8 E2 Ubc12 on a surface that overlaps the E1-binding site. PMID: 18206966
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YLR128W

    STRING: 4932.YLR128W



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