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  4. Recombinant Saccharomyces cerevisiae DNA replication regulator DPB11 (DPB11), partial

Recombinant Saccharomyces cerevisiae DNA replication regulator DPB11 (DPB11), partial

  • 中文名稱：
    釀酒酵母DPB11重組蛋白
  • 貨號(hào)：
    CSB-YP344426SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母DPB11重組蛋白
  • 貨號(hào)：
    CSB-EP344426SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母DPB11重組蛋白
  • 貨號(hào)：
    CSB-EP344426SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母DPB11重組蛋白
  • 貨號(hào)：
    CSB-BP344426SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母DPB11重組蛋白
  • 貨號(hào)：
    CSB-MP344426SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DPB11
  • Uniprot No.：
  • 別名：
    DPB11; YJL090C; J0918DNA replication regulator DPB11
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Has a role in the initiation of DNA replication. Required at S-phase checkpoint. Required for the association of PSF1 with origins. Also required for the proper activation of RAD53 in response to DNA damage and replication blocks. Multicopy suppressor of DPB2 mutation. Overexpression restores the growth defect conferred by POL2 mutation.
  • 基因功能參考文獻(xiàn)：
    1. The ddc2-S4 mutation decreases phosphorylation of histone H2A more significantly than the absence of Ddc1 and Dpb11 function does. PMID: 24586187
    2. Depletion of TopBP1/Dpb11 led to an accumulation of chromatin bridges. PMID: 24379413
    3. the inter-BRCT region of Dpb11 is a functionally conserved GINS interaction domain that is important for the initiation of DNA replication PMID: 23629628
    4. Dpb11 plays distinct roles in replication, checkpoint response and recombination processes, thereby contributing to chromosomal stability PMID: 21130053
    5. CDK promotes the formation of a newly identified fragile complex, the preloading complex (pre-LC) containing DNA polymerase epsilon (Pol epsilon), GINS, Sld2, and Dpb11 PMID: 20231317
    6. Phosphorylation of canonical motifs in Sld2 does not play a direct role in complex formation with Dpb11, but rather regulates phosphorylation of another residue, Thr84. PMID: 16619031
    7. Dpb11 functions with Ddc1 in the recombination repair process. PMID: 16840526
    8. Dpb11 is held in proximity to damaged DNA through an interaction with the phosphorylated 9-1-1 complex, leading to Mec1-dependent phosphorylation of Rad9. PMID: 18541674
    9. MSA1 is a high copy suppressor of a drc1-1 mutant. PMID: 18948746

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  • 亞細(xì)胞定位：
    Nucleus.
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YJL090C

    STRING: 4932.YJL090C



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