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  4. Recombinant Saccharomyces cerevisiae DNA repair and recombination protein RDH54 (RDH54), partial

Recombinant Saccharomyces cerevisiae DNA repair and recombination protein RDH54 (RDH54), partial

  • 中文名稱：
    釀酒酵母RDH54重組蛋白
  • 貨號：
    CSB-YP334366SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RDH54重組蛋白
  • 貨號：
    CSB-EP334366SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RDH54重組蛋白
  • 貨號：
    CSB-EP334366SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RDH54重組蛋白
  • 貨號：
    CSB-BP334366SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RDH54重組蛋白
  • 貨號：
    CSB-MP334366SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RDH54
  • Uniprot No.：
  • 別名：
    RDH54; TID1; YBR073W; YBR0715; DNA repair and recombination protein RDH54; RAD homolog 54; Recombination factor TID1; Two hybrid interaction with DMC1 protein 1) [Includes: DNA topoisomerase; EC 5.99.1.-); Putative helicase; EC 3.6.4.12)]
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in the recombinational repair of double-strand breaks (DSB) in DNA during mitosis and meiosis. Has DNA dependent ATPase activity. Promotes D-loop (displacement loop) formation with RAD51 recombinase. Modifies the topology of double-stranded DNA during the D-loop reaction to facilitate the invasion of the homologous duplex molecule by the initiating single-stranded DNA substrate. Required for adaptation from G2/M checkpoint arrest induced by a double strand break, by participating in monitoring the extent of single-stranded DNA produced by resection of DNA ends. This role is distinct from its roles in recombination. Promotes colocalization of RAD51 and DMC1 during meiotic recombination. Involved in crossover interference.
  • 基因功能參考文獻：
    1. Rdh54, a chromatin remodeler was identified as the first protein required for template switching that does not affect simple gene conversion. PMID: 25066232
    2. Data indicate that the Rad51-binding domain of Rdh54 resides within its N terminus and is required for Rad51 removal from DNA. PMID: 23798704
    3. Tid1 is a novel target of the DNA damage checkpoint pathway. PMID: 23473644
    4. Rad51-Rad54 function together to promote intersister DNA strand exchange, whereas Dmc1-Tid1 tilt the bias toward interhomolog DNA strand exchange. PMID: 22761450
    5. Rad54 and Rdh54 have different in vivo interactions with Rad51. PMID: 21558173
    6. In the absence of the Rad54, Rdh54, and Uls1 translocases, Rad51 accumulates spontaneously on chromatin, blocking cell growth and increasing genomic instability. The translocases dissociate Rad51 from sites of nonspecific dsDNA binding during mitosis. PMID: 20864034
    7. Rdh54p presumably operates via chromatin remodulation at the site of damage rendering DNA accessible to the DNA repair enzymes. PMID: 20297653
    8. Results provide molecular information concerning the role of Rdh54 in homologous recombination and DNA repair, and they also demonstrate the functional significance of Rdh54.Rad51 complex formation. PMID: 16831867
    9. Dmc1 can associate with chromatin in the absence of DNA double-strand breaks, and Tid1 suppresses this association. PMID: 16980587
    10. Results demonstrate translocation of Rdh54 on DNA and extrusion of DNA loops by Rdh54 in an ATP hydrolysis-dependent manner. PMID: 17467735
    11. translocation by Tid1 on DNA can result in disruption of three-stranded DNA structures PMID: 17704061
    12. Rdh54 has a role in chromatin rearrangement during DNA recombination and repair PMID: 18292093

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 數據庫鏈接：

    KEGG: sce:YBR073W

    STRING: 4932.YBR073W



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