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  4. Recombinant Saccharomyces cerevisiae DNA polymerase IV (POL4), partial

Recombinant Saccharomyces cerevisiae DNA polymerase IV (POL4), partial

  • 中文名稱：
    釀酒酵母POL4重組蛋白
  • 貨號：
    CSB-YP340715SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母POL4重組蛋白
  • 貨號：
    CSB-EP340715SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母POL4重組蛋白
  • 貨號：
    CSB-EP340715SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母POL4重組蛋白
  • 貨號：
    CSB-BP340715SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母POL4重組蛋白
  • 貨號：
    CSB-MP340715SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    POL4
  • Uniprot No.：
  • 別名：
    POL4; POLX; YCR014C; YCR14CDNA polymerase IV; POL IV; EC 2.7.7.7
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Repair polymerase. Involved in gap-filling in DNA nonhomologous end joining (NHEJ) required for double-strand break repair. Seems to conduct DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Preferentially acts upon short gaps formed by the alignment of linear duplexes with complementary single-strand ends. Required for filling gaps that need removal of a 5'- or 3'-terminal mismatch, however lacks nuclease activities.
  • 基因功能參考文獻：
    1. Our data suggest that the role of Tel1 in preventing break-induced chromosomal translocations can, to some extent, be due to its stimulating effect on gap-filling activity of Pol4 to repair DSBs in cis PMID: 23874240
    2. both yeast and human pol eta synthesize past the 3'-(m)C CPD in a >99% error-free manner, consistent with the highly water-exposed nature of the active site. PMID: 22262850
    3. Results describe a series of pair-wise interactions among FEN-1, Pol4 and Dnl4, the factors that complete the repair of DNA double-strand breaks by non-homologous end-joining. PMID: 15342630
    4. possible role for pol IV in base excision repair PMID: 15778218
    5. Pol4 is exclusively associated with the NHEJ pathway of double-stranded break repair PMID: 15964833
    6. Pol4 is able to extend a 3'-end whose last bases are mismatched to the template strand PMID: 16452137
    7. model predicts that a unique function of Pol4 is to actively stabilize template strands that lack stacking continuity PMID: 17881298

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-X family
  • 數據庫鏈接：

    KEGG: sce:YCR014C

    STRING: 4932.YCR014C



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