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  4. Recombinant Saccharomyces cerevisiae DNA damage-responsive transcriptional repressor RPH1 (RPH1), partial

Recombinant Saccharomyces cerevisiae DNA damage-responsive transcriptional repressor RPH1 (RPH1), partial

  • 中文名稱：
    釀酒酵母RPH1重組蛋白
  • 貨號：
    CSB-YP331103SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RPH1重組蛋白
  • 貨號：
    CSB-EP331103SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RPH1重組蛋白
  • 貨號：
    CSB-EP331103SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RPH1重組蛋白
  • 貨號：
    CSB-BP331103SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RPH1重組蛋白
  • 貨號：
    CSB-MP331103SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RPH1
  • Uniprot No.：
  • 別名：
    RPH1; YER169W; DNA damage-responsive transcriptional repressor RPH1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcriptional repressor of photolyase PHR1. Recognizes and binds the sequence AG(4) in the upstream repressing sequence of PHR1. Derepresses PHR1 transcription when phosphorylated.
  • 基因功能參考文獻：
    1. led to the identification of Rph1 as a master transcriptional regulator of autophagy PMID: 25751780
    2. Rph1/KDM4 mediates nutrient-limitation signaling that leads to the transcriptional induction of autophagy. PMID: 25660547
    3. activity of Rph1 is not essential for its role in the transcriptional response to nutrient signaling PMID: 24999627
    4. Our findings confirm the regulatory role of Rph1 as a transcriptional repressor and reveal that Rph1 might be a regulatory node connecting different signaling pathways responding to environmental stresses. PMID: 23985319
    5. these results show that of the JmjC-containing proteins, Rph1 is the demethylase that is specific for histone H3K36 trimethylation during transcription elongation in vivo. PMID: 21684259
    6. Results demonstrates that the histone demethylase Rph1 is associated with a specific chromatin locus and modulates histone modifications to repress a DNA damage responsive gene under control of damage checkpoint signaling. PMID: 21296759
    7. Results identify Rph1, a JmjC-domain-containing histone demethylase, which can specifically demethylate H3K36 tri- and dimethyl modification states. PMID: 17371840

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  • 亞細胞定位：
    Nucleus.
  • 數據庫鏈接：

    KEGG: sce:YER169W

    STRING: 4932.YER169W



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